論文 - 詳細
| RRC ID | 78879 |
|---|---|
| 著者 | Tsuchiya M, Tachibana N, Hamachi I. |
| タイトル | Post-click labeling enables highly accurate single cell analyses of glucose uptake ex vivo and in vivo. |
| ジャーナル | Commun Biol |
| Abstract |
Cellular glucose uptake is a key feature reflecting metabolic demand of cells in physiopathological conditions. Fluorophore-conjugated sugar derivatives are widely used for monitoring glucose transporter (GLUT) activity at the single-cell level, but have limitations in in vivo applications. Here, we develop a click chemistry-based post-labeling method for flow cytometric measurement of glucose uptake with low background adsorption. This strategy relies on GLUT-mediated uptake of azide-tagged sugars, and subsequent intracellular labeling with a cell-permeable fluorescent reagent via a copper-free click reaction. Screening a library of azide-substituted monosaccharides, we discover 6-azido-6-deoxy-D-galactose (6AzGal) as a suitable substrate of GLUTs. 6AzGal displays glucose-like physicochemical properties and reproduces in vivo dynamics similar to 18F-FDG. Combining this method with multi-parametric immunophenotyping, we demonstrate the ability to precisely resolve metabolically-activated cells with various GLUT activities in ex vivo and in vivo models. Overall, this method provides opportunities to dissect the heterogenous metabolic landscape in complex tissue environments. |
| 巻・号 | 7(1) |
| ページ | 459 |
| 公開日 | 2024-4-16 |
| DOI | 10.1038/s42003-024-06164-y |
| PII | 10.1038/s42003-024-06164-y |
| PMID | 38627603 |
| PMC | PMC11021395 |
| MeSH | Azides* / chemistry Glucose* / metabolism Single-Cell Analysis |
| IF | 4.165 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | K562(RCB0027) |