論文 - 詳細
| RRC ID | 78881 |
|---|---|
| 著者 | Kato K, Habu Y, Takaku H. |
| タイトル | Non-sequence specific RNA inhibition of HIV-1 replication. |
| ジャーナル | Nucleic Acids Symp Ser (Oxf) |
| Abstract |
In this study, we investigated an RNA (R-Psi-sgRNA) that suppresses HIV-1 replication. This RNA is expressed by a plasmid vector (pR-Psi-sgRNA-ter) that was constructed accidentally. To examine if this effect is caused by RNA interference, R-Psi-sgRNA was synthesized in vitro and treated with the Dicer enzyme, an important RNase III enzyme for RNA interference. The RNA was cleaved into fragments of approximately 20 nucleotides. We then performed an HIV-1 p24 assay with the RNA fragments to evaluate their effect on HIV-1 replication. HIV-1 replication was suppressed. We are now analyzing the sequences of the RNA fragments. |
| 巻・号 | (51) |
| ページ | 411-2 |
| 公開日 | 2007-1-1 |
| DOI | 10.1093/nass/nrm206 |
| PII | 51/1/411 |
| PMID | 18029761 |
| MeSH | Anti-HIV Agents / chemistry* Anti-HIV Agents / metabolism Base Sequence HIV-1 / physiology* HeLa Cells Humans Molecular Sequence Data RNA Interference* RNA, Small Untranslated Ribonuclease III / metabolism Virus Replication* |
| リソース情報 | |
| ヒト・動物細胞 | EL4(RCB1641) |