| RRC ID |
78925
|
| 著者 |
Ito K, Matsuura K, Mihara Y, Sakamoto Y, Hasegawa K, Kokudo N, Shimizu T.
|
| タイトル |
Delivery of pancreatic digestive enzymes into the gastrointestinal tract by pancreatic exocrine tissue transplant.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Exocrine pancreatic insufficiency, caused by disease-induced loss of pancreatic exocrine cells, may be treated through regenerative stem cell technologies that facilitate the production of pancreatic exocrine cells from induced pluripotent stem cells (iPSCs). However, delivering the digestive enzymes produced in the transplanted cells to the gastrointestinal tract remains a challenge. To generate an allogenic transplantation rat model, minced pancreas was transplanted into the gastric submucosal space with ablation of muscularis mucosa. In the allogenic transplantation, transplanted pancreatic cells were engrafted. Elevated amylase was detected in gastric juice, while transplanted cells disappeared through auto-digestion when the muscularis mucosa was not eliminated. Human iPSCs were differentiated into pancreatic exocrine cells by stage-specific treatment with growth factors and chemical compounds, and the differentiated pancreatic cells were implanted into the gastric submucosal space of nude rats. The transplanted cells were engrafted, and amylase was detected in the gastric juice in some cases. These findings suggest that transplantation of pancreatic exocrine cells into the gastric submucosal space with muscularis mucosa elimination will contribute to a regenerative approach for pancreatic exocrine insufficiency.
|
| 巻・号 |
9(1)
|
| ページ |
5922
|
| 公開日 |
2019-4-11
|
| DOI |
10.1038/s41598-019-42362-z
|
| PII |
10.1038/s41598-019-42362-z
|
| PMID |
30976035
|
| PMC |
PMC6459827
|
| MeSH |
Amylases / metabolism*
Animals
Cell Differentiation*
Exocrine Pancreatic Insufficiency / enzymology
Exocrine Pancreatic Insufficiency / pathology
Exocrine Pancreatic Insufficiency / therapy*
Gastric Mucosa / enzymology
Gastrointestinal Tract / enzymology*
Gastrointestinal Tract / pathology
Induced Pluripotent Stem Cells / cytology*
Male
Pancreas, Exocrine / cytology*
Pancreas, Exocrine / enzymology
Pancreas, Exocrine / surgery
Rats
Rats, Inbred F344
Rats, Nude
Stem Cell Transplantation / methods*
|
| リソース情報 |
| ヒト・動物細胞 |
201B7(HPS0063) |
