RRC ID 79001
Author Yoshiki Matsuura, Kana Kaizuka, Yoshihiro H. Inoue
Title Essential Role of COPII Proteins in Maintaining the Contractile Ring Anchoring to the Plasma Membrane during Cytokinesis in Drosophila Male Meiosis
Journal International Journal of Molecular Sciences
Abstract Coatomer Protein Complex-II (COPII) mediates anterograde vesicle transport from the endoplasmic reticulum (ER) to the Golgi apparatus. Here, we report that the COPII coatomer complex is constructed dependent on a small GTPase, Sar1, in spermatocytes before and during Drosophila male meiosis. COPII-containing foci co-localized with transitional endoplasmic reticulum (tER)-Golgi units. They showed dynamic distribution along astral microtubules and accumulated around the spindle pole, but they were not localized on the cleavage furrow (CF) sites. The depletion of the four COPII coatomer subunits, Sec16, or Sar1 that regulate COPII assembly resulted in multinucleated cell production after meiosis, suggesting that cytokinesis failed in both or either of the meiotic divisions. Although contractile actomyosin and anilloseptin rings were formed once plasma membrane ingression was initiated, they were frequently removed from the plasma membrane during furrowing. We explored the factors conveyed toward the CF sites in the membrane via COPII-mediated vesicles. DE-cadherin-containing vesicles were formed depending on Sar1 and were accumulated in the cleavage sites. Furthermore, COPII depletion inhibited de novo plasma membrane insertion. These findings suggest that COPII vesicles supply the factors essential for the anchoring and/or constriction of the contractile rings at cleavage sites during male meiosis in Drosophila.
Volume 25
Pages 4526
Published 2024-4-20
DOI 10.3390/ijms25084526
PMID 38674111
PMC PMC11050551
MeSH Animals COP-Coated Vesicles* / metabolism Cadherins / metabolism Cell Membrane* / metabolism Cytokinesis* / physiology Drosophila / metabolism Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster / metabolism Endoplasmic Reticulum* / metabolism Golgi Apparatus / metabolism Male Meiosis* / physiology Monomeric GTP-Binding Proteins / genetics Monomeric GTP-Binding Proteins / metabolism Spermatocytes / metabolism
Drosophila DGRC#109007