RRC ID 79006
Author Tsuyama T, Hayashi Y, Komai H, Shimono K, Uemura T.
Title Dynamic de novo adipose tissue development during metamorphosis in Drosophila melanogaster.
Journal Development
Abstract Adipose tissue is a central organ for controlling systemic metabolism both in invertebrates and vertebrates. Here, we have investigated the developmental processes of the adult-type fat body (AFB) in Drosophila. We have established genetic tools that allow visualization and genetic manipulations of cells in the AFB lineage from early in metamorphosis. We identified precursor cells that give rise to the AFB and delineated dynamic cellular behaviors underlying AFB formation. These precursor cells displayed polarized cell shapes and oriented motility, with emigration from the thorax and subsequent dispersal to the abdomen and head. After the migration period, these cells adhered to each other, assembling into the AFB with a sheet-like architecture. Continuous cell proliferation occurred during and after the large-scale migration to achieve appropriate fat tissue mass. Homotypic cell fusion after the sheet formation contributed to the establishment of multinucleated cells in the AFB. We also examined candidate gene functions, and our results argue that ecdysone signaling and the transcription factor Serpent support adult fat body organogenesis.
Volume 150(10)
Published 2023-5-15
DOI 10.1242/dev.200815
PII 310416
PMID 37092314
MeSH Adipose Tissue / metabolism Animals Drosophila / metabolism Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster* / genetics Drosophila melanogaster* / metabolism Ecdysone / metabolism Gene Expression Regulation, Developmental Larva / metabolism Metamorphosis, Biological / genetics Transcription Factors / metabolism