RRC ID 79097
Author Labbaf Z, Petratou K, Ermlich L, Backer W, Tarbashevich K, Reichman-Fried M, Luschnig S, Schulte-Merker S, Raz E.
Title A robust and tunable system for targeted cell ablation in developing embryos.
Journal Dev Cell
Abstract Cell ablation is a key method in the research fields of developmental biology, tissue regeneration, and tissue homeostasis. Eliminating specific cell populations allows for characterizing interactions that control cell differentiation, death, behavior, and spatial organization of cells. Current methodologies for inducing cell death suffer from relatively slow kinetics, making them unsuitable for analyzing rapid events and following primary and immediate consequences of the ablation. To address this, we developed a cell-ablation system that is based on bacterial toxin/anti-toxin proteins and enables rapid and cell-autonomous elimination of specific cell types and organs in zebrafish embryos. A unique feature of this system is that it uses an anti-toxin, which allows for controlling the degree and timing of ablation and the resulting phenotypes. The transgenic zebrafish generated in this work represent a highly efficient tool for cell ablation, and this approach is applicable to other model organisms as demonstrated here for Drosophila.
Volume 57(16)
Pages 2026-2040.e5
Published 2022-8-22
DOI 10.1016/j.devcel.2022.07.008
PII S1534-5807(22)00497-X
PMID 35914525
MeSH Animals Animals, Genetically Modified Cell Death Cell Differentiation Drosophila* Zebrafish* / genetics
IF 10.092
Zebrafish Tg(sox17:EGFP)ha01