論文 - 詳細
| RRC ID | 79097 |
|---|---|
| 著者 | Labbaf Z, Petratou K, Ermlich L, Backer W, Tarbashevich K, Reichman-Fried M, Luschnig S, Schulte-Merker S, Raz E. |
| タイトル | A robust and tunable system for targeted cell ablation in developing embryos. |
| ジャーナル | Dev Cell |
| Abstract |
Cell ablation is a key method in the research fields of developmental biology, tissue regeneration, and tissue homeostasis. Eliminating specific cell populations allows for characterizing interactions that control cell differentiation, death, behavior, and spatial organization of cells. Current methodologies for inducing cell death suffer from relatively slow kinetics, making them unsuitable for analyzing rapid events and following primary and immediate consequences of the ablation. To address this, we developed a cell-ablation system that is based on bacterial toxin/anti-toxin proteins and enables rapid and cell-autonomous elimination of specific cell types and organs in zebrafish embryos. A unique feature of this system is that it uses an anti-toxin, which allows for controlling the degree and timing of ablation and the resulting phenotypes. The transgenic zebrafish generated in this work represent a highly efficient tool for cell ablation, and this approach is applicable to other model organisms as demonstrated here for Drosophila. |
| 巻・号 | 57(16) |
| ページ | 2026-2040.e5 |
| 公開日 | 2022-8-22 |
| DOI | 10.1016/j.devcel.2022.07.008 |
| PII | S1534-5807(22)00497-X |
| PMID | 35914525 |
| MeSH | Animals Animals, Genetically Modified Cell Death Cell Differentiation Drosophila* Zebrafish* / genetics |
| IF | 10.092 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(sox17:EGFP)ha01 |