論文 - 詳細
| RRC ID | 79155 |
|---|---|
| 著者 | Inomata M, Abe M, Kawase Y, Hayashi T, Amano S, Sakagami H. |
| タイトル | Dectin-1/SYK Activation Induces Antimicrobial Peptide and Negative Regulator of NF-κB Signaling in Human Oral Epithelial Cells. |
| ジャーナル | In Vivo |
| Abstract |
BACKGROUND/AIM:Oral epithelial cells serve as the primary defense against microbial exposure in the oral cavity, including the fungus Candida albicans. Dectin-1 is crucial for recognition of β-glucan in fungi. However, expression and function of Dectin-1 in oral epithelial cells remain unclear. MATERIALS AND METHODS:We assessed Dectin-1 expression in Ca9-22 (gingiva), HSC-2 (mouth), HSC-3 (tongue), and HSC-4 (tongue) human oral epithelial cells using flow cytometry and real-time polymerase chain reaction. Cell treated with β-glucan-rich zymosan were evaluated using real-time polymerase chain reaction. Phosphorylation of spleen-associated tyrosine kinase (SYK) was analyzed by western blotting. RESULTS:Dectin-1 was expressed in all four cell types, with high expression in Ca9-22 and HSC-2. In Ca9-22 cells, exposure to β-glucan-rich zymosan did not alter the mRNA expression of chemokines nor of interleukin (IL)6, IL8, IL1β, IL17A, and IL17F. Zymosan induced the expression of antimicrobial peptides β-defensin-1 and LL-37, but not S100 calcium-binding protein A8 (S100A8) and S100A9. Furthermore, the expression of cylindromatosis (CYLD), a negative regulator of nuclear factor kappa B (NF-κB) signaling, was induced. In HSC-2 cells, zymosan induced the expression of IL17A. The expression of tumor necrosis factor alpha-induced protein 3 (TNFAIP3), a negative regulator of NF-κB signaling, was also induced. Expression of other cytokines and antimicrobial peptides remained unchanged. Zymosan induced phosphorylation of SYK in Ca9-22 cells, as well as NF-κB. CONCLUSION:Oral epithelial cells express Dectin-1 and recognize β-glucan, which activates SYK and induces the expression of antimicrobial peptides and negative regulators of NF-κB, potentially maintaining oral homeostasis. |
| 巻・号 | 38(3) |
| ページ | 1042-1048 |
| 公開日 | 2024-1-1 |
| DOI | 10.21873/invivo.13537 |
| PII | 38/3/1042 |
| PMID | 38688646 |
| PMC | PMC11059888 |
| MeSH | Antimicrobial Cationic Peptides / genetics Antimicrobial Cationic Peptides / metabolism Cell Line Cytokines / genetics Cytokines / metabolism Epithelial Cells* / drug effects Epithelial Cells* / metabolism Humans Lectins, C-Type* / genetics Lectins, C-Type* / metabolism Mouth Mucosa / immunology Mouth Mucosa / metabolism NF-kappa B* / metabolism Phosphorylation Pore Forming Cytotoxic Proteins / genetics Pore Forming Cytotoxic Proteins / metabolism Signal Transduction* Syk Kinase* / genetics Syk Kinase* / metabolism Zymosan / pharmacology |
| リソース情報 | |
| ヒト・動物細胞 | Ca9-22(RCB1976) HSC-2(RCB1945) HSC-3(RCB1975) HSC-4(RCB1902) |