| RRC ID |
79181
|
| Author |
Suhara Y, Wada A, Tachibana Y, Watanabe M, Nakamura K, Nakagawa K, Okano T.
|
| Title |
Structure-activity relationships in the conversion of vitamin K analogues into menaquinone-4. Substrates essential to the synthesis of menaquinone-4 in cultured human cell lines.
|
| Journal |
Bioorg Med Chem
|
| Abstract |
To reveal an essential biological role of menaquinone-4, we have clarified that dietary PK was converted to menaquinone-4 (MK-4) in animal tissues using deuterated vitamin K analogues. However, the kinds of analogue converted into MK-4 have not been elucidated. In this study, we examined structure-activity relationships in the conversion of several vitamin K analogues, with a substituted side chain, into MK-4 using cultured human cell lines. The results differed with the side chain of the analogues, that is, (1) the length of the isoprene unit and (2) the number of double bonds in the side chain. These findings would be useful for clarifying the mechanism of conversion of other vitamin K homologs into MK-4 as well as related enzymes.
|
| Volume |
18(9)
|
| Pages |
3116-24
|
| Published |
2010-5-1
|
| DOI |
10.1016/j.bmc.2010.03.035
|
| PII |
S0968-0896(10)00239-7
|
| PMID |
20378364
|
| MeSH |
Cell Line
Cell Line, Tumor
Chromatography, High Pressure Liquid
Hemostatics / chemical synthesis
Hemostatics / chemistry
Humans
Mass Spectrometry
Molecular Structure
Structure-Activity Relationship
Substrate Specificity
Vitamin K / analogs & derivatives
Vitamin K / chemistry*
Vitamin K 2 / analogs & derivatives*
Vitamin K 2 / chemical synthesis
Vitamin K 2 / chemistry
|
| Resource |
| Human and Animal Cells |
MG-63(RCB1890) |
