RRC ID 79210
Author Tanimoto Y, Kakinuma H, Aoki R, Shiraki T, Higashijima SI, Okamoto H.
Title Transgenic tools targeting the basal ganglia reveal both evolutionary conservation and specialization of neural circuits in zebrafish.
Journal Cell Rep
Abstract The cortico-basal ganglia circuit mediates decision making. Here, we generated transgenic tools for adult zebrafish targeting specific subpopulations of the components of this circuit and utilized them to identify evolutionary homologs of the mammalian direct- and indirect-pathway striatal neurons, which respectively project to the homologs of the internal and external segment of the globus pallidus (dorsal entopeduncular nucleus [dEN] and lateral nucleus of the ventral telencephalic area [Vl]) as in mammals. Unlike in mammals, the Vl mainly projects to the dEN directly, not by way of the subthalamic nucleus. Further single-cell RNA sequencing analysis reveals two pallidal output pathways: a major shortcut pathway directly connecting the dEN with the pallium and the evolutionarily conserved closed loop by way of the thalamus. Our resources and circuit map provide the common basis for the functional study of the basal ganglia in a small and optically tractable zebrafish brain for the comprehensive mechanistic understanding of the cortico-basal ganglia circuit.
Volume 43(3)
Pages 113916
Published 2024-3-26
DOI 10.1016/j.celrep.2024.113916
PII S2211-1247(24)00244-4
PMID 38484735
MeSH Animals Animals, Genetically Modified Basal Ganglia* / physiology Corpus Striatum Globus Pallidus / physiology Mammals Neural Pathways / physiology Zebrafish* / genetics
IF 8.109
Resource
Zebrafish Tg(UAS:GFP)nkuasgfp1aTg Tg(UAS:AcGFP-2A-WGA)rw0318Tg Tg(hspa8:Cre-mCherry-NLS)nns65Tg TgBAC(tac1:GAL4VP16)rw157 Tg(tac1:zGFF)rw158, Tg(penkb:zGFF)rw159 TgBAC(nkx2.1:GAL4VP16)rw160 TgBAC(npy:GAL4VP16)rw161b TgBAC(gad1b:GAL4VP16)rw162b Tg(pitx2-hs:loxP-mCherry-loxP-zGFF) Tg(crhb-hs:loxP-RFP-loxP-GFP) Tg(crhb-hs:GFP)