| RRC ID |
79210
|
| 著者 |
Tanimoto Y, Kakinuma H, Aoki R, Shiraki T, Higashijima SI, Okamoto H.
|
| タイトル |
Transgenic tools targeting the basal ganglia reveal both evolutionary conservation and specialization of neural circuits in zebrafish.
|
| ジャーナル |
Cell Rep
|
| Abstract |
The cortico-basal ganglia circuit mediates decision making. Here, we generated transgenic tools for adult zebrafish targeting specific subpopulations of the components of this circuit and utilized them to identify evolutionary homologs of the mammalian direct- and indirect-pathway striatal neurons, which respectively project to the homologs of the internal and external segment of the globus pallidus (dorsal entopeduncular nucleus [dEN] and lateral nucleus of the ventral telencephalic area [Vl]) as in mammals. Unlike in mammals, the Vl mainly projects to the dEN directly, not by way of the subthalamic nucleus. Further single-cell RNA sequencing analysis reveals two pallidal output pathways: a major shortcut pathway directly connecting the dEN with the pallium and the evolutionarily conserved closed loop by way of the thalamus. Our resources and circuit map provide the common basis for the functional study of the basal ganglia in a small and optically tractable zebrafish brain for the comprehensive mechanistic understanding of the cortico-basal ganglia circuit.
|
| 巻・号 |
43(3)
|
| ページ |
113916
|
| 公開日 |
2024-3-26
|
| DOI |
10.1016/j.celrep.2024.113916
|
| PII |
S2211-1247(24)00244-4
|
| PMID |
38484735
|
| MeSH |
Animals
Animals, Genetically Modified
Basal Ganglia* / physiology
Corpus Striatum
Globus Pallidus / physiology
Mammals
Neural Pathways / physiology
Zebrafish* / genetics
|
| IF |
8.109
|
| リソース情報 |
| ゼブラフィッシュ |
Tg(UAS:GFP)nkuasgfp1aTg
Tg(UAS:AcGFP-2A-WGA)rw0318Tg
Tg(hspa8:Cre-mCherry-NLS)nns65Tg
TgBAC(tac1:GAL4VP16)rw157
Tg(tac1:zGFF)rw158, Tg(penkb:zGFF)rw159
TgBAC(nkx2.1:GAL4VP16)rw160
TgBAC(npy:GAL4VP16)rw161b
TgBAC(gad1b:GAL4VP16)rw162b
Tg(pitx2-hs:loxP-mCherry-loxP-zGFF)
Tg(crhb-hs:loxP-RFP-loxP-GFP)
Tg(crhb-hs:GFP) |
