RRC ID 79211
Author Xu C, Xu J, Tang HW, Ericsson M, Weng JH, DiRusso J, Hu Y, Ma W, Asara JM, Perrimon N.
Title A phosphate-sensing organelle regulates phosphate and tissue homeostasis.
Journal Nature
Abstract Inorganic phosphate (Pi) is one of the essential molecules for life. However, little is known about intracellular Pi metabolism and signalling in animal tissues1. Following the observation that chronic Pi starvation causes hyperproliferation in the digestive epithelium of Drosophila melanogaster, we determined that Pi starvation triggers the downregulation of the Pi transporter PXo. In line with Pi starvation, PXo deficiency caused midgut hyperproliferation. Interestingly, immunostaining and ultrastructural analyses showed that PXo specifically marks non-canonical multilamellar organelles (PXo bodies). Further, by Pi imaging with a Förster resonance energy transfer (FRET)-based Pi sensor2, we found that PXo restricts cytosolic Pi levels. PXo bodies require PXo for biogenesis and undergo degradation following Pi starvation. Proteomic and lipidomic characterization of PXo bodies unveiled their distinct feature as an intracellular Pi reserve. Therefore, Pi starvation triggers PXo downregulation and PXo body degradation as a compensatory mechanism to increase cytosolic Pi. Finally, we identified connector of kinase to AP-1 (Cka), a component of the STRIPAK complex and JNK signalling3, as the mediator of PXo knockdown- or Pi starvation-induced hyperproliferation. Altogether, our study uncovers PXo bodies as a critical regulator of cytosolic Pi levels and identifies a Pi-dependent PXo-Cka-JNK signalling cascade controlling tissue homeostasis.
Volume 617(7962)
Pages 798-806
Published 2023-5-1
DOI 10.1038/s41586-023-06039-y
PII 10.1038/s41586-023-06039-y
PMID 37138087
PMC PMC10443203
MeSH Adaptor Proteins, Signal Transducing / metabolism Animals Cytosol / metabolism Drosophila Proteins / deficiency Drosophila Proteins / metabolism Drosophila melanogaster* / anatomy & histology Drosophila melanogaster* / cytology Drosophila melanogaster* / metabolism Fluorescence Resonance Energy Transfer Homeostasis* JNK Mitogen-Activated Protein Kinases / metabolism Lipidomics Organelles* / metabolism Phosphates* / deficiency Phosphates* / metabolism Proteomics
Resource
Drosophila 10483R-3