| RRC ID |
79219
|
| 著者 |
Rayamajhi D, Ege M, Ukhanov K, Ringers C, Zhang Y, Jung I, D'Gama PP, Li SS, Cosacak MI, Kizil C, Park HC, Yaksi E, Martens JR, Brody SL, Jurisch-Yaksi N, Roy S.
|
| タイトル |
The forkhead transcription factor Foxj1 controls vertebrate olfactory cilia biogenesis and sensory neuron differentiation.
|
| ジャーナル |
PLoS Biol
|
| Abstract |
In vertebrates, olfactory receptors localize on multiple cilia elaborated on dendritic knobs of olfactory sensory neurons (OSNs). Although olfactory cilia dysfunction can cause anosmia, how their differentiation is programmed at the transcriptional level has remained largely unexplored. We discovered in zebrafish and mice that Foxj1, a forkhead domain-containing transcription factor traditionally linked with motile cilia biogenesis, is expressed in OSNs and required for olfactory epithelium (OE) formation. In keeping with the immotile nature of olfactory cilia, we observed that ciliary motility genes are repressed in zebrafish, mouse, and human OSNs. Strikingly, we also found that besides ciliogenesis, Foxj1 controls the differentiation of the OSNs themselves by regulating their cell type-specific gene expression, such as that of olfactory marker protein (omp) involved in odor-evoked signal transduction. In line with this, response to bile acids, odors detected by OMP-positive OSNs, was significantly diminished in foxj1 mutant zebrafish. Taken together, our findings establish how the canonical Foxj1-mediated motile ciliogenic transcriptional program has been repurposed for the biogenesis of immotile olfactory cilia, as well as for the development of the OSNs.
|
| 巻・号 |
22(1)
|
| ページ |
e3002468
|
| 公開日 |
2024-1-1
|
| DOI |
10.1371/journal.pbio.3002468
|
| PII |
PBIOLOGY-D-23-01574
|
| PMID |
38271330
|
| PMC |
PMC10810531
|
| MeSH |
Animals
Cilia / metabolism
Forkhead Transcription Factors / genetics
Forkhead Transcription Factors / metabolism
Humans
Mice
Olfactory Mucosa
Olfactory Receptor Neurons*
Zebrafish* / genetics
Zebrafish* / metabolism
|
| IF |
7.076
|
| リソース情報 |
| ゼブラフィッシュ |
Tg(OMP:Gal4FF)rw0320
UAS:GCaMP6s |
