RRC ID |
79222
|
Author |
Chuma S, Kiyosue K, Akiyama T, Kinoshita M, Shimazaki Y, Uchiyama S, Sotoma S, Okabe K, Harada Y.
|
Title |
Implication of thermal signaling in neuronal differentiation revealed by manipulation and measurement of intracellular temperature.
|
Journal |
Nat Commun
|
Abstract |
Neuronal differentiation-the development of neurons from neural stem cells-involves neurite outgrowth and is a key process during the development and regeneration of neural functions. In addition to various chemical signaling mechanisms, it has been suggested that thermal stimuli induce neuronal differentiation. However, the function of physiological subcellular thermogenesis during neuronal differentiation remains unknown. Here we create methods to manipulate and observe local intracellular temperature, and investigate the effects of noninvasive temperature changes on neuronal differentiation using neuron-like PC12 cells. Using quantitative heating with an infrared laser, we find an increase in local temperature (especially in the nucleus) facilitates neurite outgrowth. Intracellular thermometry reveals that neuronal differentiation is accompanied by intracellular thermogenesis associated with transcription and translation. Suppression of intracellular temperature increase during neuronal differentiation inhibits neurite outgrowth. Furthermore, spontaneous intracellular temperature elevation is involved in neurite outgrowth of primary mouse cortical neurons. These results offer a model for understanding neuronal differentiation induced by intracellular thermal signaling.
|
Volume |
15(1)
|
Pages |
3473
|
Published |
2024-5-9
|
DOI |
10.1038/s41467-024-47542-8
|
PII |
10.1038/s41467-024-47542-8
|
PMID |
38724563
|
PMC |
PMC11082174
|
MeSH |
Animals
Cell Differentiation*
Mice
Neural Stem Cells / cytology
Neural Stem Cells / metabolism
Neural Stem Cells / physiology
Neurites / metabolism
Neurites / physiology
Neurogenesis / physiology
Neuronal Outgrowth
Neurons* / cytology
Neurons* / physiology
PC12 Cells
Rats
Signal Transduction*
Temperature*
Thermogenesis / physiology
Thermometry / methods
|
Resource |
Human and Animal Cells |
PC-12(RCB0009) |