RRC ID 79222
Author Chuma S, Kiyosue K, Akiyama T, Kinoshita M, Shimazaki Y, Uchiyama S, Sotoma S, Okabe K, Harada Y.
Title Implication of thermal signaling in neuronal differentiation revealed by manipulation and measurement of intracellular temperature.
Journal Nat Commun
Abstract Neuronal differentiation-the development of neurons from neural stem cells-involves neurite outgrowth and is a key process during the development and regeneration of neural functions. In addition to various chemical signaling mechanisms, it has been suggested that thermal stimuli induce neuronal differentiation. However, the function of physiological subcellular thermogenesis during neuronal differentiation remains unknown. Here we create methods to manipulate and observe local intracellular temperature, and investigate the effects of noninvasive temperature changes on neuronal differentiation using neuron-like PC12 cells. Using quantitative heating with an infrared laser, we find an increase in local temperature (especially in the nucleus) facilitates neurite outgrowth. Intracellular thermometry reveals that neuronal differentiation is accompanied by intracellular thermogenesis associated with transcription and translation. Suppression of intracellular temperature increase during neuronal differentiation inhibits neurite outgrowth. Furthermore, spontaneous intracellular temperature elevation is involved in neurite outgrowth of primary mouse cortical neurons. These results offer a model for understanding neuronal differentiation induced by intracellular thermal signaling.
Volume 15(1)
Pages 3473
Published 2024-5-9
DOI 10.1038/s41467-024-47542-8
PII 10.1038/s41467-024-47542-8
PMID 38724563
PMC PMC11082174
MeSH Animals Cell Differentiation* Mice Neural Stem Cells / cytology Neural Stem Cells / metabolism Neural Stem Cells / physiology Neurites / metabolism Neurites / physiology Neurogenesis / physiology Neuronal Outgrowth Neurons* / cytology Neurons* / physiology PC12 Cells Rats Signal Transduction* Temperature* Thermogenesis / physiology Thermometry / methods
Resource
Human and Animal Cells PC-12(RCB0009)