| RRC ID |
79368
|
| 著者 |
Chen L, Zhang M, Li X, Wu Q, Xue X, Zhang T, Lu R, Zhang Y.
|
| タイトル |
AphA directly activates the transcription of polysaccharide biosynthesis gene scvE in Vibrio parahaemolyticus.
|
| ジャーナル |
Gene
|
| Abstract |
Vibrio parahaemolyticus, a seafood-borne pathogen, is capable of forming biofilms on surfaces. Exopolysaccharide (EPS) plays crucial roles in holding bacterial cells together and keeping biofilm attached on the surface. The cpsA-K and scvA-O gene clusters are responsible for EPS synthesis in V. parahaemolyticus. AphA, the master quorum sensing (QS) regulator operating at low cell density (LCD), positively regulates transcription of cpsA-K and scvA-O, but lacks the detailed mechanisms. The present data showed that the aphA mutant produced smooth colonies, whereas the wild-type strain produced wrinkled colonies. AphA bound the regulatory DNA region of scvE to activate its transcription, whereas it positively regulated transcription of cpsA and scvA in an indirect manner. The transcriptional level of scvE gradually decreased with increasing cell density, which correlated with the expression level of aphA. Taken together, this work elucidated how AphA regulated the biofilm-associated colony morphology variation in V. parahaemolyticus through its regulatory actions on the expression of EPS genes.
|
| 巻・号 |
851
|
| ページ |
146980
|
| 公開日 |
2023-1-30
|
| DOI |
10.1016/j.gene.2022.146980
|
| PII |
S0378-1119(22)00800-9
|
| PMID |
36261093
|
| MeSH |
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Biofilms
Gene Expression Regulation, Bacterial
Polysaccharides / genetics
Polysaccharides / metabolism
Quorum Sensing / genetics
Vibrio parahaemolyticus* / metabolism
|
| IF |
2.984
|
| リソース情報 |
| 病原細菌 |
JNBP_30766 |
