論文 - 詳細
| RRC ID | 79392 |
|---|---|
| 著者 | Getz LJ, Brown JM, Sobot L, Chow A, Mahendrarajah J, Thomas NA. |
| タイトル | Attenuation of a DNA cruciform by a conserved regulator directs T3SS1 mediated virulence in Vibrio parahaemolyticus. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Pathogenic Vibrio species account for 3-5 million annual life-threatening human infections. Virulence is driven by bacterial hemolysin and toxin gene expression often positively regulated by the winged helix-turn-helix (wHTH) HlyU transcriptional regulator family and silenced by histone-like nucleoid structural protein (H-NS). In the case of Vibrio parahaemolyticus, HlyU is required for virulence gene expression associated with type 3 Secretion System-1 (T3SS1) although its mechanism of action is not understood. Here, we provide evidence for DNA cruciform attenuation mediated by HlyU binding to support concomitant virulence gene expression. Genetic and biochemical experiments revealed that upon HlyU mediated DNA cruciform attenuation, an intergenic cryptic promoter became accessible allowing for exsA mRNA expression and initiation of an ExsA autoactivation feedback loop at a separate ExsA-dependent promoter. Using a heterologous E. coli expression system, we reconstituted the dual promoter elements which revealed that HlyU binding and DNA cruciform attenuation were strictly required to initiate the ExsA autoactivation loop. The data indicate that HlyU acts to attenuate a transcriptional repressive DNA cruciform to support T3SS1 virulence gene expression and reveals a non-canonical extricating gene regulation mechanism in pathogenic Vibrio species. |
| 巻・号 | 51(12) |
| ページ | 6156-6171 |
| 公開日 | 2023-7-7 |
| DOI | 10.1093/nar/gkad370 |
| PII | 7157527 |
| PMID | 37158250 |
| PMC | PMC10325908 |
| MeSH | Bacterial Proteins / metabolism DNA, Cruciform / metabolism Escherichia coli / genetics Gene Expression Regulation, Bacterial Humans Type III Secretion Systems / genetics Vibrio parahaemolyticus* / genetics Vibrio parahaemolyticus* / metabolism Virulence / genetics |
| IF | 11.502 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原細菌 | JNBP_30766 |