論文 - 詳細
| RRC ID | 79394 |
|---|---|
| 著者 | Liu F, Wang F, Yuan Y, Li X, Zhong X, Yang M. |
| タイトル | Quorum sensing signal synthases enhance Vibrio parahaemolyticus swarming motility. |
| ジャーナル | Mol Microbiol |
| Abstract |
Vibrio parahaemolyticus is a significant food-borne pathogen that is found in diverse aquatic habitats. Quorum sensing (QS), a signaling system for cell-cell communication, plays an important role in V. parahaemolyticus persistence. We characterized the function of three V. parahaemolyticus QS signal synthases, CqsAvp , LuxMvp , and LuxSvp , and show that they are essential to activate QS and regulate swarming. We found that CqsAvp , LuxMvp , and LuxSvp activate a QS bioluminescence reporter through OpaR. However, V. parahaemolyticus exhibits swarming defects in the absence of CqsAvp , LuxMvp , and LuxSvp , but not OpaR. The swarming defect of this synthase mutant (termed Δ3AI) was recovered by overexpressing either LuxOvpD47A , a mimic of dephosphorylated LuxOvp mutant, or the scrABC operon. CqsAvp , LuxMvp , and LuxSvp inhibit lateral flagellar (laf) gene expression by inhibiting the phosphorylation of LuxOvp and the expression of scrABC. Phosphorylated LuxOvp enhances laf gene expression in a mechanism that involves modulating c-di-GMP levels. However, enhancing swarming requires phosphorylated and dephosphorylated LuxOvp which is regulated by the QS signals that are synthesized by CqsAvp , LuxMvp , and LuxSvp . The data presented here suggest an important strategy of swarming regulation by the integration of QS and c-di-GMP signaling pathways in V. parahaemolyticus. |
| 巻・号 | 120(2) |
| ページ | 241-257 |
| 公開日 | 2023-8-1 |
| DOI | 10.1111/mmi.15113 |
| PMID | 37330634 |
| MeSH | Bacterial Proteins / genetics Bacterial Proteins / metabolism Gene Expression Regulation, Bacterial Quorum Sensing* / genetics Signal Transduction Vibrio parahaemolyticus* / physiology |
| IF | 3.418 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原細菌 | |