RRC ID 79418
Author Makihira S, Yan W, Ohno S, Kawamoto T, Fujimoto K, Okimura A, Yoshida E, Noshiro M, Hamada T, Kato Y.
Title Enhancement of cell adhesion and spreading by a cartilage-specific noncollagenous protein, cartilage matrix protein (CMP/Matrilin-1), via integrin alpha1beta1.
Journal J Biol Chem
Abstract Cartilage matrix protein (CMP; also known as matrilin-1), one of the major noncollagenous proteins in most cartilages, binds to aggrecan and type II collagen. We examined the effect of CMP on the adhesion of chondrocytes and fibroblasts using CMP-coated dishes. The CMP coating at 10-20 micrograms/ml enhanced the adhesion and spreading of rabbit growth plate, resting and articular chondrocytes, and fibroblasts and human epiphyseal chondrocytes and MRC5 fibroblasts. The effect of CMP on the spreading of chondrocytes was synergistically increased by native, but not heated, type II collagen (gelatin). The monoclonal antibody to integrin alpha1 or beta1 abolished CMP-induced cell adhesion and spreading, whereas the antibody to integrin alpha2, alpha3, alpha5, beta2, alpha5beta1, or alphaVbeta5 had little effect on cell adhesion or spreading. The antibody to integrin alpha1, but not to other subunits, coprecipitated 125I-CMP that was added to MRC5 cell lysates, indicating the association of CMP with the integrin alpha1 subunit. Unlabeled CMP competed for the binding to integrin alpha1 with 125I-CMP. These findings suggest that CMP is a potent adhesion factor for chondrocytes, particularly in the presence of type II collagen, and that integrin alpha1beta1 is involved in CMP-mediated cell adhesion and spreading. Since CMP is expressed almost exclusively in cartilage, this adhesion factor, unlike fibronectin or laminin, may play a special role in the development and remodeling of cartilage.
Volume 274(16)
Pages 11417-23
Published 1999-4-16
DOI 10.1074/jbc.274.16.11417
PII S0021-9258(19)73654-0
PMID 10196235
MeSH Animals Antibodies / immunology Cartilage Cartilage Oligomeric Matrix Protein Cell Adhesion / physiology Cell Movement / physiology Collagen / metabolism Extracellular Matrix Proteins / metabolism Extracellular Matrix Proteins / physiology* Female Glycoproteins / metabolism Glycoproteins / physiology* Humans Integrin alpha1beta1 Integrins / immunology Integrins / physiology* Matrilin Proteins Mice Mice, Inbred BALB C Protein Binding Rabbits Swine
IF 4.238
Human and Animal Cells MRC-5