Reference - Detail
| RRC ID | 79448 |
|---|---|
| Author | Minamitani T, Ariga H, Matsumoto K. |
| Title | Adhesive defect in extracellular matrix tenascin-X-null fibroblasts: a possible mechanism of tumor invasion. |
| Journal | Biol Pharm Bull |
| Abstract |
Extracellular matrix tenascin-X (TNX)-null mice, generated by disruption of the Tnx gene, display augmented invasion and metastasis of B16-BL6 melanoma tumor cells due to increased activities of matrix metalloproteinase (MMP)-2 and MMP-9. In this study, we investigated cell-matrix and cell-cell adhesions using TNX-null fibroblasts and wild-type fibroblasts. TNX-null fibroblasts exhibited a decreased attachment to fibronectin compared with that of wild-type fibroblasts. B16 melanoma cells were cocultured with wild-type or TNX-null fibroblasts, and the adhesion of B16 melanoma to the fibroblasts was assessed. B16 melanoma cells on wild-type fibroblasts proliferated and spread out in a horizontal direction, whereas those on TNX-null fibroblasts overlapped each other rather than migrating horizontally. These overlapping B16 melanoma cells on TNX-null fibroblasts peeled off faster than those on wild-type fibroblasts. To determine whether the decreased cell-matrix and cell-cell adhesions on TNX-null fibroblasts were due to increased MMP activity, the activities of MMPs in wild-type and TNX-null fibroblasts were compared by gelatinolytic assays. The analysis of MMPs from conditioned media demonstrated that almost the same levels of MMP activities were detected between wild-type and TNX-null fibroblasts. However, contrary to our expectations the activities of MMPs from conditioned media of B16 melanoma cells cocultured on TNX-null fibroblasts were rather reduced than those of B16 melanoma cells cocultured on wild-type. We concluded that the absence of TNX in the extracellular environment might play an important role in enhancement of the detachment of B16 melanoma cells. |
| Volume | 25(11) |
| Pages | 1472-5 |
| Published | 2002-11-1 |
| DOI | 10.1248/bpb.25.1472 |
| PMID | 12419962 |
| MeSH | Animals Cell Adhesion / genetics Coculture Techniques / methods Extracellular Matrix / genetics Extracellular Matrix / metabolism* Fibroblasts / metabolism* Melanoma, Experimental / genetics Melanoma, Experimental / metabolism* Melanoma, Experimental / pathology* Mice Mice, Inbred C57BL Mice, Inbred CBA Mice, Inbred ICR Mice, Knockout Neoplasm Invasiveness / genetics Neoplasm Invasiveness / pathology Tenascin / deficiency* Tenascin / genetics* Tumor Cells, Cultured |
| IF | 1.863 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | B16 melanoma/lacZ(RCB1284) |