論文 - 詳細
| RRC ID | 79491 |
|---|---|
| 著者 | Goto T, Sasaki T, Chong Y, Taniguchi M, Lee JM, Masuda A, Ebihara T, Shiraishi K, Tani N, Yonekawa A, Gondo K, Kuwano H, Shimono N, Ikematsu H, Akashi K, Kusakabe T. |
| タイトル | SARS-CoV-2 strain-specific anti-spike IgG ELISA utilizing spike protein produced by silkworms. |
| ジャーナル | Hum Antibodies |
| Abstract |
BACKGROUND:A cost-effective and eco-friendly method is needed for the assessment of humoral immunity against SARS-CoV-2 in large populations. OBJECTIVE:We investigated the performance of an ELISA that uses silkworm-produced proteins to quantify the strain-specific anti-Spike IgG (anti-S IgG) titer. METHODS:The OD values for the anti-His-tag antibody, a standard material of ELISA quantification, were measured. Correlations between the ELISA for each strain and the Abbott SARS-CoV-2 IgG II Quant assay for the wild type were evaluated with serum samples from nine participants with various infection and vaccination statuses. RESULTS:Linear dose-responses were confirmed by high coefficients of determination: 0.994, 0.994, and 0.996 for the wild-type, Delta, and Omicron (BA.1) strain assays, respectively. The coefficient of determination for the wild-type and Delta strain assays was high at 0.959 and 0.892, respectively, while the Omicron strain assay had a relatively low value of 0.563. Booster vaccinees showed similar or higher titers against all strains compared to infected persons without vaccination. The Omicron-infected persons without vaccination had lower antibody titers against wild type than did the vaccinated persons. CONCLUSIONS:This study provides data indicating that the ELISA with silkworm-produced proteins makes it possible to discriminate and quantify the strain-specific anti-S IgG antibody induced by vaccination or infection. |
| 巻・号 | 31(3) |
| ページ | 27-33 |
| 公開日 | 2023-1-1 |
| DOI | 10.3233/HAB-230006 |
| PII | HAB230006 |
| PMID | 37458030 |
| MeSH | Animals Antibodies, Neutralizing Antibodies, Viral Bombyx* COVID-19* Enzyme-Linked Immunosorbent Assay Humans Immunoglobulin G SARS-CoV-2 Spike Glycoprotein, Coronavirus |
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