論文 - 詳細
| RRC ID | 79656 |
|---|---|
| 著者 | Matsui S, Kataoka H, Tanaka JI, Kikuchi M, Fukamachi H, Morisaki H, Matsushima H, Mishima K, Hironaka S, Takaki T, Okahashi N, Maruoka Y, Kuwata H. |
| タイトル | Dysregulation of Intestinal Microbiota Elicited by Food Allergy Induces IgA-Mediated Oral Dysbiosis. |
| ジャーナル | Infect Immun |
| Abstract |
Food allergy is a life-threatening response to specific foods, and microbiota imbalance (dysbiosis) in gut is considered a cause of this disease. Meanwhile, the host immune response also plays an important role in the disease. Notably, interleukin 33 (IL-33) released from damaged or necrotic intestinal epithelial cells facilitates IL-2-producing CD4 helper T (Th2) responses. However, causal relationships between the gut and oral dysbiosis and food allergy remain unknown. In this study, we analyzed effects of gut and oral dysbiosis on development of food allergy. A murine model of food allergy was established via ovalbumin (OVA) injection in BALB/c mice. Viable fecal bacteria were identified using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). il33 expression in colon-26 mouse colon cells stimulated by isolated fecal bacteria was quantified by real-time PCR. Intestinal T cells from the mice were analyzed by flow cytometry. Salivary IgA levels were quantified by enzyme-linked immunosorbent assay (ELISA), and IgA-bound oral bacteria were detected by flow cytometry. Among fecal bacteria, the abundance of Citrobacter sp. increased in the feces of allergic mice and induced il33 expression in colon-26 cells. Orally administered Citrobacter koseri JCM1658 exacerbated systemic allergic symptoms and reduced intestinal Th17 cells. Salivary IgA and IgA-bound oral bacteria increased in the allergic mice. Based on the results described above, food allergy induced both gut and oral dysbiosis. Citrobacter sp. aggravated allergy symptoms by inducing IL-33 release from intestinal epithelial cells. |
| 巻・号 | 88(1) |
| 公開日 | 2019-12-17 |
| DOI | 10.1128/IAI.00741-19 |
| PII | IAI.00741-19 |
| PMID | 31611274 |
| PMC | PMC6921656 |
| MeSH | Animals Cytokines / metabolism Disease Models, Animal Dysbiosis* Epithelial Cells / metabolism Food Hypersensitivity / complications* Gastrointestinal Tract / microbiology* Immunoglobulin A / metabolism* Immunologic Factors / metabolism* Mice, Inbred BALB C Microbiota / drug effects* Mouth / microbiology* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 1658 |
| ヒト・動物細胞 | Colon-26(RCB2657) |