| RRC ID |
80036
|
| Author |
Ninomiya K, Kaneda K, Kawashima S, Miyachi Y, Ogino C, Shimizu N.
|
| Title |
Cell-SELEX based selection and characterization of DNA aptamer recognizing human hepatocarcinoma.
|
| Journal |
Bioorg Med Chem Lett
|
| Abstract |
Single-stranded DNA aptamers recognizing human hepatocarcinoma were isolated by means of a systematic evolution of ligands by exponential enrichment using whole cells as targets (cell-SELEX). After 11 rounds of cell-SELEX procedure using human hepatoma HepG2 cells as targets and human normal hepatocyte cells as counterparts, 12 independent DNA aptamer candidate sequences were obtained. The specific interaction between selected DNA aptamers and targeted cell was confirmed. Dissociation constants of the 12 sequences obtained were also estimated in the range of 19-450nM. Moreover, the consensus secondary structure was found in the isolated aptamers, which was responsible to the recognition of HepG2 cells.
|
| Volume |
23(6)
|
| Pages |
1797-802
|
| Published |
2013-3-15
|
| DOI |
10.1016/j.bmcl.2013.01.040
|
| PII |
S0960-894X(13)00073-5
|
| PMID |
23403083
|
| MeSH |
Aptamers, Nucleotide / chemistry*
Aptamers, Nucleotide / metabolism
Base Sequence
Carcinoma, Hepatocellular
Cell Separation*
DNA, Single-Stranded / chemistry
DNA, Single-Stranded / metabolism*
Flow Cytometry
Hep G2 Cells
Humans
Liver Neoplasms
Microscopy, Fluorescence
Nucleic Acid Conformation
SELEX Aptamer Technique
|
| IF |
2.572
|
| Resource |
| Human and Animal Cells |
Hep G2(RCB1648) |
