RRC ID 80078
Author Kajihara R, Ezaki R, Ichikawa K, Watanabe T, Terada T, Matsuzaki M, Horiuchi H.
Title Wnt signaling blockade is essential for maintaining the pluripotency of chicken embryonic stem cells.
Journal Poult Sci
Abstract Activation of Wnt/β-catenin signaling supports the self-renewal of mouse embryonic stem cells. We aimed to understand the effects of Wnt signaling activation or inhibition on chicken embryonic stem cells (chESCs), as these effects are largely unknown. When the glycogen synthase kinase-3 β inhibitor CHIR99021-which activates Wnt signaling-was added to chESC cultures, the colony shape flattened, and the expression levels of pluripotency-related (NANOG, SOX2, SOX3, OCT4, LIN28A, DNMT3B, and PRDM14) and germ cell (CVH and DAZL) markers showed a decreasing trend, and the growth of chESCs was inhibited after approximately 7 d. By contrast, when the Wnt signaling inhibitor XAV939 was added to the culture, dense and compact multipotent colonies (morphologically similar to mouse embryonic stem cell colonies) showing stable expression of pluripotency-related and germline markers were formed. The addition of XAV939 stabilized the proliferation of chESCs in the early stages of culture and promoted their establishment. Furthermore, these chESCs formed chimeras. In conclusion, functional chESCs can be stably cultured using Wnt signaling inhibitors. These findings suggest the importance of Wnt/β-catenin signaling in avian stem cells, offering valuable insights for applied research using chESCs.
Volume 103(2)
Pages 103361
Published 2024-2-1
DOI 10.1016/j.psj.2023.103361
PII S0032-5791(23)00881-7
PMID 38154448
PMC PMC10788285
MeSH Animals Cell Differentiation Chickens* / metabolism Embryonic Stem Cells / metabolism Mice Wnt Signaling Pathway* beta Catenin / metabolism
IF 2.659
Resource
Chicken / Quail White Leghorn