Reference - Detail
| RRC ID | 80091 |
|---|---|
| Author | Sekine S, Shimada Y, Nagata T, Moriyama M, Omura T, Yoshioka I, Hori R, Matsui K, Sawada S, Okumura T, Yoshida T, Tsukada K. |
| Title | Establishment and characterization of a new human gallbladder carcinoma cell line. |
| Journal | Anticancer Res |
| Abstract |
BACKGROUND:Prognosis for patients with gallbladder carcinoma (GBC) is poor and the standard treatment for GBC has not yet been established. MATERIALS AND METHODS:We established the human GBC cell line TYGBK-1, from a patient with papillary, tubular adenocarcinoma. RESULTS:The doubling time was 48 hours. This cell line has a missense mutation of p53 and no mutation of the K-RAS gene. This cell line was transplantable to nude mice. We characterized the sensitivity of TYGBK-1 to gemcitabine. We also examined the association of two gemcitabine-related genes (deoxycytidine kinase, dCK, and Hu antigen R, HuR). Among four GBC cell lines (TYGBK-1, NOZ, G-415, TGBC2TKB), TYGBK-1 and NOZ exhibited sensitivity to gemcitabine. Furthermore, these cells expressed both dCK and HuR mRNA, rather than gemcitabine-resistant cells. CONCLUSION:The newly established GBC cell line TYGBK-1, may represent an effective tool for development of chemotherapeutic treatment for GBC. |
| Volume | 32(8) |
| Pages | 3211-8 |
| Published | 2012-8-1 |
| PII | 32/8/3211 |
| PMID | 22843894 |
| MeSH | Adenocarcinoma / genetics Adenocarcinoma / pathology* Adenocarcinoma, Papillary / genetics Adenocarcinoma, Papillary / pathology Aged Animals Base Sequence Cell Line, Tumor DNA Primers Female Gallbladder Neoplasms / genetics Gallbladder Neoplasms / pathology* Genes, p53 Genes, ras Humans Immunohistochemistry Mice Mice, Nude Mutation, Missense Real-Time Polymerase Chain Reaction |
| IF | 1.994 |
| Resource | |
| Human and Animal Cells | G-415(RCB2640) TGBC2TKB(RCB1130) |