論文 - 詳細
| RRC ID | 80170 |
|---|---|
| 著者 | Nii T, Maeda Y, Motooka D, Naito M, Matsumoto Y, Ogawa T, Oguro-Igashira E, Kishikawa T, Yamashita M, Koizumi S, Kurakawa T, Okumura R, Kayama H, Murakami M, Sakaguchi T, Das B, Nakamura S, Okada Y, Kumanogoh A, Takeda K. |
| タイトル | Genomic repertoires linked with pathogenic potency of arthritogenic Prevotella copri isolated from the gut of patients with rheumatoid arthritis. |
| ジャーナル | Ann Rheum Dis |
| Abstract |
OBJECTIVES:Prevotella copri is considered to be a contributing factor in rheumatoid arthritis (RA). However, in some non-Westernised countries, healthy individuals also harbour an abundance of P. copri in the intestine. This study investigated the pathogenicity of RA patient-derived P. copri (P. copriRA) compared with healthy control-derived P. copri (P. copriHC). METHODS:We obtained 13 P. copri strains from the faeces of patients with RA and healthy controls. Following whole genome sequencing, the sequences of P. copriRA and P. copriHC were compared. To analyse the arthritis-inducing ability of P. copri, we examined two arthritis models (1) a collagen-induced arthritis model harbouring P. copri under specific-pathogen-free conditions and (2) an SKG mouse arthritis model under P. copri-monocolonised conditions. Finally, to evaluate the ability of P. copri to activate innate immune cells, we performed in vitro stimulation of bone marrow-derived dendritic cells (BMDCs) by P. copriRA and P. copriHC. RESULTS:Comparative genomic analysis revealed no apparent differences in the core gene contents between P. copriRA and P. copriHC, but pangenome analysis revealed the high genome plasticity of P. copri. We identified a P. copriRA-specific genomic region as a conjugative transposon. In both arthritis models, P. copriRA-induced more severe arthritis than P. copriHC. In vitro BMDC stimulation experiments revealed the upregulation of IL-17 and Th17-related cytokines (IL-6, IL-23) by P. copriRA. CONCLUSION:Our findings reveal the genetic diversity of P. copri, and the genomic signatures associated with strong arthritis-inducing ability of P. copriRA. Our study contributes towards elucidation of the complex pathogenesis of RA. |
| 巻・号 | 82(5) |
| ページ | 621-629 |
| 公開日 | 2023-5-1 |
| DOI | 10.1136/ard-2022-222881 |
| PII | S0003-4967(24)08461-9 |
| PMID | 36627170 |
| PMC | PMC10176341 |
| MeSH | Animals Arthritis, Rheumatoid* / genetics Disease Models, Animal Gastrointestinal Microbiome* / genetics Genomics Mice Prevotella / genetics |
| リソース情報 | |
| 一般微生物 | JCM 13464 |