| RRC ID |
80190
|
| 著者 |
Joshi A, Thite S, Lodha T, Joseph N, Mengade P.
|
| タイトル |
Genomic insights of an alkaliphilic bacterium Halalkalibacter alkaliphilus sp. nov. isolated from an Indian Soda Lake.
|
| ジャーナル |
Antonie Van Leeuwenhoek
|
| Abstract |
An alkaliphilic, Gram-stain-positive, non-motile, rod-shaped, and spore forming bacterial strain (MEB205T) was isolated from sediment sample collected from Lonar lake, India. The strain grew optimally at pH 10, NaCl concentration of 3.0% at 37 °C. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain MEB205T belonged to the genus Halalkalibacter in the family Bacillaceae and shared the highest sequence similarity with H. okhensis Kh10-101T (98.9%) followed by H. wakoensis N-1 T (98.7%). The assembled genome of strain MEB205T has a total length of 4.8 Mb with a G + C content of 37.8%. The dDDH and OrthoANI values between strain MEB205T and H. okhensis Kh10-101 T were 29.1% and 84.3%, respectively. Furthermore, the genome analysis revealed the presence of antiporter genes (nhaA and nhaD) and L-ectoine biosynthesis gene required for survival of the strain MEB205T in alkaline-saline habitat. The major fatty acid was C15:0 anteiso, C16:0 and C15:0 iso (> 10.0%). Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were the major polar lipids. meso-diaminopimelic acid was diagnostic diamino acid for cell wall peptidoglycan. Based on the polyphasic taxonomic studies, strain MEB205T represent a novel species of the genus Halalkalibacter for which the name Halalkalibacter alkaliphilus sp. nov. (Type strain MEB205T = MCC 3863 T = JCM 34004 T = NCIMB 15406 T) is proposed.
|
| 巻・号 |
116(5)
|
| ページ |
435-445
|
| 公開日 |
2023-5-1
|
| DOI |
10.1007/s10482-023-01816-1
|
| PII |
10.1007/s10482-023-01816-1
|
| PMID |
36811745
|
| MeSH |
Bacterial Typing Techniques
DNA, Bacterial / genetics
Fatty Acids / analysis
Genomics
Lakes* / microbiology
Nucleic Acid Hybridization
Phospholipids* / analysis
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| リソース情報 |
| 一般微生物 |
JCM 34004 |
