| RRC ID |
80214
|
| Author |
Dengler L, Meier J, Klingl A, Nißl L, Bellack A, Grohmann D, Rachel R, Huber H.
|
| Title |
A novel interdomain consortium from a Costa Rican oil well composed of Methanobacterium cahuitense sp. nov. and Desulfomicrobium aggregans sp. nov.
|
| Journal |
Arch Microbiol
|
| Abstract |
A novel interdomain consortium composed of a methanogenic Archaeon and a sulfate-reducing bacterium was isolated from a microbial biofilm in an oil well in Cahuita National Park, Costa Rica. Both organisms can be grown in pure culture or as stable co-culture. The methanogenic cells were non-motile rods producing CH4 exclusively from H2/CO2. Cells of the sulfate-reducing partner were motile rods forming cell aggregates. They utilized hydrogen, lactate, formate, and pyruvate as electron donors. Electron acceptors were sulfate, thiosulfate, and sulfite. 16S rRNA sequencing revealed 99% gene sequence similarity of strain CaP3V-M-L2AT to Methanobacterium subterraneum and 98.5% of strain CaP3V-S-L1AT to Desulfomicrobium baculatum. Both strains grew from 20 to 42 °C, pH 5.0-7.5, and 0-4% NaCl. Based on our data, type strains CaP3V-M-L2AT (= DSM 113354 T = JCM 39174 T) and CaP3V-S-L1AT (= DSM 113299 T = JCM 39179 T) represent novel species which we name Methanobacterium cahuitense sp. nov. and Desulfomicrobium aggregans sp. nov.
|
| Volume |
205(5)
|
| Pages |
189
|
| Published |
2023-4-13
|
| DOI |
10.1007/s00203-023-03533-9
|
| PII |
10.1007/s00203-023-03533-9
|
| PMID |
37055657
|
| PMC |
PMC10102059
|
| MeSH |
Costa Rica
DNA, Bacterial / genetics
Fatty Acids
Methanobacterium* / genetics
Oil and Gas Fields*
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sulfates / metabolism
|
| Resource |
| General Microbes |
JCM 39174
JCM 39179 |
