Reference - Detail
| RRC ID | 80229 |
|---|---|
| Author | Laily IN, Takeuchi M, Mizutani T, Ogawa J. |
| Title | An ACE2, SARS-CoV-2 spike protein binding protein, -like enzyme isolated from food-related microorganisms. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Angiotensin-converting enzyme 2 (ACE2) is a binding target of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein. An ACE2-like enzyme, such as bacterial M32-carboxypeptidase (M32-CAP), is assumed to be a potential therapeutic candidate for coronavirus disease 2019 (COVID-19). Here, we screened bacteria with an ACE2-like enzyme activity from Japanese fermented food and dietary products using the fluorogenic substrate for rapid screening. The strain showing the highest activity, Enterobacter sp. 200527-13, produced an enzyme with the same hydrolytic activity as ACE2 on Angiotensin II (Ang II). The enzymatic analysis using the heterologously-expressed enzyme in Escherichia coli revealed that the enzyme catalyzes the same reaction with that of ACE2, Ang II hydrolysis to Ang 1-7, and phenylalanine. The gene sequence information showed that the enzyme belongs to the M32-CAP family. These results suggested that the selected enzyme, M32-CAP (EntCP), from Enterobacter sp. 200527-13 was identified as an ACE2-like enzyme. |
| Volume | 87(6) |
| Pages | 638-645 |
| Published | 2023-5-19 |
| DOI | 10.1093/bbb/zbad037 |
| PII | 7095858 |
| PMID | 36997336 |
| MeSH | Angiotensin-Converting Enzyme 2 / metabolism COVID-19* Carrier Proteins / metabolism Humans Peptidyl-Dipeptidase A / genetics Peptidyl-Dipeptidase A / metabolism Protein Binding SARS-CoV-2 / metabolism Spike Glycoprotein, Coronavirus / chemistry Spike Glycoprotein, Coronavirus / genetics Spike Glycoprotein, Coronavirus / metabolism |
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| Total number of mentions | 1 |
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| General Microbes | JCM 18047 JCM 1168 JCM 18559 JCM 5817 JCM 11263 JCM 18191 JCM 1114 JCM 1174 |