| RRC ID |
80229
|
| Author |
Laily IN, Takeuchi M, Mizutani T, Ogawa J.
|
| Title |
An ACE2, SARS-CoV-2 spike protein binding protein, -like enzyme isolated from food-related microorganisms.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
Angiotensin-converting enzyme 2 (ACE2) is a binding target of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein. An ACE2-like enzyme, such as bacterial M32-carboxypeptidase (M32-CAP), is assumed to be a potential therapeutic candidate for coronavirus disease 2019 (COVID-19). Here, we screened bacteria with an ACE2-like enzyme activity from Japanese fermented food and dietary products using the fluorogenic substrate for rapid screening. The strain showing the highest activity, Enterobacter sp. 200527-13, produced an enzyme with the same hydrolytic activity as ACE2 on Angiotensin II (Ang II). The enzymatic analysis using the heterologously-expressed enzyme in Escherichia coli revealed that the enzyme catalyzes the same reaction with that of ACE2, Ang II hydrolysis to Ang 1-7, and phenylalanine. The gene sequence information showed that the enzyme belongs to the M32-CAP family. These results suggested that the selected enzyme, M32-CAP (EntCP), from Enterobacter sp. 200527-13 was identified as an ACE2-like enzyme.
|
| Volume |
87(6)
|
| Pages |
638-645
|
| Published |
2023-5-19
|
| DOI |
10.1093/bbb/zbad037
|
| PII |
7095858
|
| PMID |
36997336
|
| MeSH |
Angiotensin-Converting Enzyme 2 / metabolism
COVID-19*
Carrier Proteins / metabolism
Humans
Peptidyl-Dipeptidase A / genetics
Peptidyl-Dipeptidase A / metabolism
Protein Binding
SARS-CoV-2 / metabolism
Spike Glycoprotein, Coronavirus / chemistry
Spike Glycoprotein, Coronavirus / genetics
Spike Glycoprotein, Coronavirus / metabolism
|
| Resource |
| General Microbes |
JCM 18047
JCM 1168
JCM 18559
JCM 5817
JCM 11263
JCM 18191
JCM 1114
JCM 1174 |
