Reference - Detail
| RRC ID | 80230 |
|---|---|
| Author | Hui CW, Kishino S, Nakatani Y, Ogawa J. |
| Title | α-Tomatine degradation to tomatidine by food-related Aspergillus species belonging to the section Nigri. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
α-Tomatine is a steroidal glycoalkaloid in tomato plants and degrades with ripening. The aglycone form, tomatidine, is reported to have beneficial effects. In this study, the ability of food-related microorganisms to produce tomatidine from α-tomatine was evaluated. A total of 11 strains of Aspergillus species belonging to the section Nigri exhibited tomatinase activity, and Aspergillus luchuensis JCM 22302 was selected for optimization due to its high activity in its mycelia, conidia, and non-mycotoxin-producing property. Next, using A. luchuensis JCM22302 conidia, the highest yield was obtained in a 24-h reaction with 50 m m of acetic acid-sodium acetate buffer (pH 5.5) at 37 °C. Similar to the tomato pathogen Fusarium oxysporum f. lyceopersici, the time course analysis suggested that A. luchuensis JCM 22302 removed the entire sugar moiety in a single step. Future research will focus on utilizing conidia for large-scale tomatidine production because of their high tolerance and manageability. |
| Volume | 87(6) |
| Pages | 663-671 |
| Published | 2023-5-19 |
| DOI | 10.1093/bbb/zbad031 |
| PII | 7081303 |
| PMID | 36941129 |
| MeSH | Aspergillus* / metabolism Tomatine* / chemistry Tomatine* / metabolism |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 22302 JCM 5546 JCM 5549 JCM 1864 JCM 5638 JCM 22282 JCM 5635 JCM 22322 JCM 22293 JCM 22302 JCM 5628 |