RRC ID 80237
Author Nishiyama C, Sekiguchi S, Sugihara Y, Nishikawa M, Makita N, Segawa T, Terasaki M, Takagi H, Koyanagi T.
Title Phylogenomic analysis in Latilactobacillus sakei by using polymorphisms detected by next-generation sequencing.
Journal Biosci Microbiota Food Health
Abstract Latilactobacillus sakei is a lactic acid bacterium used to produce a wide range of fermented food products. To understand their characteristics and adaptability to various nutrient sources, we applied strain-specific, nucleotide-concatenated (SSC) sequences to the phylogenetic analysis of 32 L. sakei strains isolated from various locations and products. SSC sequences were developed by concatenating the polymorphisms detected by whole-genome sequencing. This enabled us to use sufficient polymorphisms and avoid the bias caused by selecting partial sequences, such as that in core genome and multi-locus sequence typing. SSC sequence-based analysis revealed that the phylogenetic relations for L. sakei are based on the different nutrition sources rather than geographical distance.
Volume 42(2)
Pages 138-142
Published 2023-1-1
DOI 10.12938/bmfh.2022-017
PII 2022-017
PMID 37016685
PMC PMC10067329
Resource
General Microbes JCM 1157 JCM 1128 JCM 1129 JCM 11031 JCM 12491