Reference - Detail
| RRC ID | 80244 |
|---|---|
| Author | Shirato M, Nakamura K, Tenkumo T, Niwano Y, Kanno T, Sasaki K, Lingström P, Örtengren U. |
| Title | Inhibition of tooth demineralization caused by Streptococcus mutans biofilm via antimicrobial treatment using hydrogen peroxide photolysis. |
| Journal | Clin Oral Investig |
| Abstract |
OBJECTIVES:An antimicrobial technique utilizing hydroxyl radicals generated by the photolysis of 3% H2O2 has been developed recently. The present study aimed to evaluate the effect of H2O2 photolysis treatment on tooth demineralization caused by Streptococcus mutans biofilm. MATERIALS AND METHODS:To induce tooth demineralization, S. mutans biofilm was allowed to form on the maxillary first molars collected from Wistar rats via 24-h culturing. The samples were immersed in 3% H2O2 and irradiated with 365-nm LED (H2O2 photolysis treatment). Viable bacterial counts in the biofilm were evaluated immediately after treatment and after an additional 30-h culturing by colony counting. The acidogenicity of the biofilm, re-established 30 h after treatment, was assessed by measuring the pH. The effect of H2O2 photolysis treatment on tooth demineralization was assessed by measuring the depth of the radiolucent layer in micro-CT images. RESULTS:H2O2 photolysis significantly reduced viable bacterial counts in the biofilm to 3.7 log colony forming units (CFU)/sample, while the untreated group had 7.9 log CFU/sample. The pH of the biofilm re-established after treatment (6.6) was higher than that of the untreated group (5.3). In line with the pH measurement, the treatment group had a significantly lower depth of radiolucent layer in dentin than the untreated group. CONCLUSIONS:H2O2 photolysis treatment was effective not only in killing the biofilm-forming S. mutans but also in lowering the acidogenicity of the biofilm. Thus, this technique could inhibit tooth demineralization. CLINICAL RELEVANCE:H2O2 photolysis can be applicable as a new dental caries treatment. |
| Volume | 27(2) |
| Pages | 739-750 |
| Published | 2023-2-1 |
| DOI | 10.1007/s00784-022-04821-2 |
| PII | 10.1007/s00784-022-04821-2 |
| PMID | 36484848 |
| PMC | PMC9889499 |
| MeSH | Animals Anti-Infective Agents* / pharmacology Biofilms Dental Caries* / microbiology Hydrogen Peroxide / pharmacology Photolysis Rats Rats, Wistar Streptococcus mutans Tooth Demineralization* / drug therapy Tooth Demineralization* / prevention & control |
| Resource | |
| General Microbes | JCM 5705 |