RRC ID |
80253
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Author |
Oikawa D, Fukui K, Aoki Y, Waki T, Takahashi S, Shimoyama T, Nakayama T.
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Title |
Enterocloster alcoholdehydrogenati sp. nov., a Novel Bacterial Species Isolated from the Feces of a Patient with Alcoholism.
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Journal |
Curr Microbiol
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Abstract |
Strain C5-48T, an anaerobic intestinal bacterium that potentially accumulates acetaldehyde at levels exceeding its minimum mutagenic concentration (50 µM) in the colon and rectum, was isolated from the feces of a patient with alcoholism. The 16S rRNA gene sequence of strain C5-48T showed high similarity to the corresponding sequences of Lachnoclostridium edouardi Marseille-P3397T (95.7%) and Clostridium fessum SNUG30386T (94.7%). However, phylogenetic analysis using the sequences of the 16S rRNA, rpoB, and hsp60 genes and whole-genome analysis strongly suggested that C5-48T should be included in the genus Enterocloster. The novelty of strain C5-48T was further confirmed by comprehensive average nucleotide identity (ANI) calculations based on its whole-genome sequence, which showed appreciable ANI values with known Enterocloster species (e.g., 74.3% and 73.4% with Enterocloster bolteae WAL 16351T and Enterocloster clostridioformis ATCC 25537T, respectively). The temperature range for growth of strain C5-48T was 15-37 °C with an optimum of 37 °C. The pH range for growth was 5.5-10.5 with an optimum of 7.5. The major constituents of the cell membrane lipids of strain C5-48T were 16:0, 14:0, and 18:1 ω7c dimethyl acetal fatty acids. On the basis of the genotypic and phenotypic properties, Enterocloster alcoholdehydrogenati sp. nov. is proposed, with the type strain C5-48T (= JCM 33305T = DSM 109474T).
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Volume |
80(5)
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Pages |
187
|
Published |
2023-4-19
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DOI |
10.1007/s00284-023-03285-1
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PII |
10.1007/s00284-023-03285-1
|
PMID |
37074467
|
PMC |
PMC10115706
|
MeSH |
Alcoholism* / microbiology
Bacteria* / classification
Bacteria* / isolation & purification
Chemotaxis
Feces* / microbiology
Phylogeny
Whole Genome Sequencing
|
Resource |
General Microbes |
JCM 33305
JCM 1291 |