RRC ID 80295
Author Hirano R, Nishita I, Nakai R, Bito A, Sasabe R, Kurihara S.
Title Development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria.
Journal Front Cell Infect Microbiol
Abstract In recent years, with the development of non-cultivation approaches, it has become evident that intestinal bacteria have a significant impact on human health. However, because one-third of the genes cannot be annotated, it is difficult to elucidate the function of all intestinal bacteria by in silico analysis, and it is necessary to study the intestinal bacteria by culturing them. In addition, various media recommended for each individual bacterium have been used for culturing intestinal bacteria; however, the preparation of each medium is complex. To simultaneously culture many bacteria and compare bacterial phenotypes under the same conditions, a medium capable of culturing a wide range of bacteria is needed. In this study, we developed GAM + blood medium (GB medium), which consists of Gifu anaerobic medium containing 5% (v/v) horse blood; it is easy to prepare and it allowed the successful cultivation of 85% of the available predominant species in the human intestinal microbiota.
Volume 13
Pages 1056866
Published 2023-1-1
DOI 10.3389/fcimb.2023.1056866
PMID 37520440
PMC PMC10374021
MeSH Animals Bacteria* / genetics Culture Media Culture Techniques Gastrointestinal Microbiome* / genetics Horses Humans
Resource
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