論文 - 詳細
| RRC ID | 80295 |
|---|---|
| 著者 | Hirano R, Nishita I, Nakai R, Bito A, Sasabe R, Kurihara S. |
| タイトル | Development of culture methods capable of culturing a wide range of predominant species of intestinal bacteria. |
| ジャーナル | Front Cell Infect Microbiol |
| Abstract |
In recent years, with the development of non-cultivation approaches, it has become evident that intestinal bacteria have a significant impact on human health. However, because one-third of the genes cannot be annotated, it is difficult to elucidate the function of all intestinal bacteria by in silico analysis, and it is necessary to study the intestinal bacteria by culturing them. In addition, various media recommended for each individual bacterium have been used for culturing intestinal bacteria; however, the preparation of each medium is complex. To simultaneously culture many bacteria and compare bacterial phenotypes under the same conditions, a medium capable of culturing a wide range of bacteria is needed. In this study, we developed GAM + blood medium (GB medium), which consists of Gifu anaerobic medium containing 5% (v/v) horse blood; it is easy to prepare and it allowed the successful cultivation of 85% of the available predominant species in the human intestinal microbiota. |
| 巻・号 | 13 |
| ページ | 1056866 |
| 公開日 | 2023-1-1 |
| DOI | 10.3389/fcimb.2023.1056866 |
| PMID | 37520440 |
| PMC | PMC10374021 |
| MeSH | Animals Bacteria* / genetics Culture Media Culture Techniques Gastrointestinal Microbiome* / genetics Horses Humans |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
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| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
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