| RRC ID |
80302
|
| 著者 |
Li S, You X, Rani A, Özcan E, Sela DA.
|
| タイトル |
Bifidobacterium infantis utilizes N-acetylglucosamine-containing human milk oligosaccharides as a nitrogen source.
|
| ジャーナル |
Gut Microbes
|
| Abstract |
Bifidobacterium longum subsp. infantis (B. infantis) utilizes oligosaccharides secreted in human milk as a carbohydrate source. These human milk oligosaccharides (HMOs) integrate the nitrogenous residue N-acetylglucosamine (NAG), although HMO nitrogen utilization has not been described to date. Herein, we characterize the B. infantis nitrogen utilization phenotype on two NAG-containing HMO species, LNT and LNnT. This was characterized through in vitro growth kinetics, incorporation of isotopically labeled NAG nitrogen into the proteome, as well as modulation of intracellular 2-oxoglutarate levels while utilizing HMO nitrogen. Further support is provided by comparative transcriptomics and proteomics that identified global regulatory networks deployed during HMO nitrogen utilization. The aggregate data demonstrate that B. infantis strains utilize HMO nitrogen with the potential to significantly impact fundamental and clinical studies, as well as enable applications.
|
| 巻・号 |
15(2)
|
| ページ |
2244721
|
| 公開日 |
2023-12-1
|
| DOI |
10.1080/19490976.2023.2244721
|
| PMID |
37609905
|
| PMC |
PMC10448974
|
| MeSH |
Acetylglucosamine
Bifidobacterium longum subspecies infantis*
Gastrointestinal Microbiome*
Humans
Milk, Human
Nitrogen
Oligosaccharides
|
| リソース情報 |
| 一般微生物 |
JCM 1260
JCM 7009 |
