RRC ID 80404
著者 Fukano H, Kazumi Y, Sakagami N, Fujiwara N, Ato M, Mitarai S, Hoshino Y.
タイトル Mycobacterium kiyosense sp. nov., a scotochromogenic slow-glowing species isolated from respiratory specimens.
ジャーナル Int J Syst Evol Microbiol
Abstract Scotochromogenic slow-growing mycobacteria were isolated from the sputum or bronchoalveolar lavage fluid of 12 patients in Japan. From a comparison of the whole-genome sequences, the representative strain IWGMT90018-18076T and the unknown strains obtained from the patients were found to represent a novel species related to the Mycobacterium gordonae complex. The average nucleotide identity values of IWGMT90018-18076T with Mycobacterium vicinigordonae, Mycobacterium paragordonae and M. gordonae were 86.7, 82.5 and 82.2 %, respectively. The genome size of the representative strain IWGMT90018-18076T was approximately 6.3 Mbp, and the genomic DNA G+C content was 67.1 %. The major fatty acid methyl esters were C16 : 0 (37.71 %), C18 : 1ω9c (29.5 %) and C16 : 1ω7c (10.32 %). In this study, we performed phylogenetic analyses, physiological and biochemical characteristic tests, drug susceptibility tests and fatty acid profiling of the clinical isolates. On the basis of the results obtained, we propose that the unknown clinical isolates represent a novel species, 'Mycobacterium kiyosense sp. nov,' with the type strain being IWGMT90018-18076T (=JCM 34837T =KCTC 49725T).
巻・号 73(6)
公開日 2023-6-1
DOI 10.1099/ijsem.0.005917
PMID 37339072
MeSH Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Fatty Acids* / chemistry Humans Mycobacterium* Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
リソース情報
一般微生物 JCM 34837 JCM 6367 JCM 18585 JCM 6379