| Abstract |
A Gram-stain-negative, rod-shaped, non-motile and strictly aerobic bacterium, which showed biofilm-forming ability on polystyrene, designated as strain B-399T, was isolated from the estuarine sediment of the Arakawa River near Tokyo Bay. It grew at pH 6.0-8.5, at 15-35 °C and in the presence of 0-7.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain B-399T was clustered in the genus Sinisalibacter and has 96.94 % sequence similarity to Sinisalibacter lacisalsi X12M-4T, which was the only validly described species in this genus. On the basis of our genome sequencing analyses, the average nucleotide identity and digital DNA-DNA hybridization values between strains B-399T and S. lacisalsi X12M-4T were 79.54 and 22.30 %, respectively, which confirms that strain B-399T represents a novel species of the genus Sinisalibacter. The draft genome size and the DNA G+C content of strain B-399T were 4.12 Mb and 65.2 mol%, respectively. The major fatty acids (>10 %) of strain B-399T were C16 : 0, summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C19 : 0 cyclo ω8c. The polar lipids were phosphatidylcholine, phosphatidylglycerol, an unidentified phospholipid, an unidentified aminolipid and unidentified lipids. The respiratory quinone was Q-10. These chemotaxonomic features were almost coincident with those of the genus Sinisalibacter. Therefore, strain B-399T should be classified as representing a new species of the genus Sinisalibacter, for which the name Sinisalibacter aestuarii sp. nov. is proposed. The type strain is B-399T (=NBRC 115629T=DSM 114148T).
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