RRC ID 80471
Author Liang Y, Shi W, Wu LH, Zhang XJ, Chai LJ, Liu GQ, Zheng L, Wang ST, Zhang SY, Chen FW, Shen CH, Xu ZH, Lu ZM.
Title Solibacillus daqui sp. nov., isolated from high-temperature Daqu.
Journal Int J Syst Evol Microbiol
Abstract A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterial strain, designated ZS111008T, was isolated from high-temperature Daqu, a starter for production of Chinese Jiang-flavour Baijiu, and was characterized by polyphasic taxonomy. This novel isolate grew in the presence of 0-5 % (w/v) NaCl, at pH 6.0-9.0 and 25-45 °C; optimum growth was observed with 1 % (w/v) NaCl, at pH 8.0 and 30 °C. A comparative analysis of the 16S rRNA gene sequence (1461 bp) of strain ZS111008T showed highest similarity to Solibacillus silvestris DSM12223T (96.7%), followed by Solibacillus cecembensis PN5T (96.6%) and Solibacillus isronensis AMCK01000046 (96.5%). The DNA G+C content of strain ZS111008T was 37.21 mol%. The respiratory quinone was identified as menaquinone-7 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and one unknown phospholipid. Lys was detected as the diagnostic diamino acid in the cell wall. Based on morphological characteristics, chemotaxonomic characteristics and physiological properties, strain ZS111008T represents a novel species of the genus Solibacillus, for which the name Solibacillus daqui sp. nov. is proposed. The type strain for this proposed species is ZS111008T (=CGMCC 1.19455T=JCM 35214T).
Volume 73(11)
Published 2023-11-1
DOI 10.1099/ijsem.0.006109
PMID 37916708
MeSH Bacterial Typing Techniques Base Composition China DNA, Bacterial / genetics Fatty Acids* / chemistry Phospholipids / chemistry Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sodium Chloride* Temperature
General Microbes JCM 35214