| RRC ID |
80502
|
| 著者 |
Song Y, Huang R, Li L, Du K, Zhu F, Song C, Yuan X, Wang M, Wang S, Ferry JG, Zhou S, Yan Z.
|
| タイトル |
Humic acid-dependent respiratory growth of Methanosarcina acetivorans involves pyrroloquinoline quinone.
|
| ジャーナル |
ISME J
|
| Abstract |
Although microbial humus respiration plays a critical role in organic matter decomposition and biogeochemical cycling of elements in diverse anoxic environments, the role of methane-producing species (methanogens) is not well defined. Here we report that a major fraction of humus, humic acid reduction enhanced the growth of Methanosarcina acetivorans above that attributed to methanogenesis when utilizing the energy sources methanol or acetate, results which showed both respiratory and fermentative modes of energy conservation. Growth characteristics with methanol were the same for an identically cultured mutant deleted for the gene encoding a multi-heme cytochrome c (MmcA), results indicating MmcA is not essential for respiratory electron transport to humic acid. Transcriptomic analyses revealed that growth with humic acid promoted the upregulation of genes annotated as cell surface pyrroloquinoline quinone (PQQ)-binding proteins. Furthermore, PQQ isolated from the membrane fraction was more abundant in humic acid-respiring cells, and the addition of PQQ improved efficiency of the extracellular electron transport. Given that the PQQ-binding proteins are widely distributed in methanogens, the findings extend current understanding of microbial humus respiration in the context of global methane dynamics.
|
| 巻・号 |
17(11)
|
| ページ |
2103-2111
|
| 公開日 |
2023-11-1
|
| DOI |
10.1038/s41396-023-01520-y
|
| PII |
10.1038/s41396-023-01520-y
|
| PMID |
37737251
|
| PMC |
PMC10579383
|
| MeSH |
Humic Substances*
Methane / metabolism
Methanol* / metabolism
Methanosarcina / metabolism
PQQ Cofactor / metabolism
|
| リソース情報 |
| 一般微生物 |
JCM 12185 |
