RRC ID 80503
Author Hirakata Y, Mei R, Morinaga K, Katayama T, Tamaki H, Meng XY, Watari T, Yamaguchi T, Hatamoto M, Nobu MK.
Title Identification and cultivation of anaerobic bacterial scavengers of dead cells.
Journal ISME J
Abstract The cycle of life and death and Earth's carbon cycle(s) are intimately linked, yet how bacterial cells, one of the largest pools of biomass on Earth, are recycled back into the carbon cycle remains enigmatic. In particular, no bacteria capable of scavenging dead cells in oxygen-depleted environments have been reported thus far. In this study, we discover the first anaerobes that scavenge dead cells and the two isolated strains use distinct strategies. Based on live-cell imaging, transmission electron microscopy, and hydrolytic enzyme assays, one strain (designated CYCD) relied on cell-to-cell contact and cell invagination for degrading dead food bacteria where as the other strain (MGCD) degraded dead food bacteria via excretion of lytic extracellular enzymes. Both strains could degrade dead cells of differing taxonomy (bacteria and archaea) and differing extents of cell damage, including those without artificially inflicted physical damage. In addition, both depended on symbiotic metabolic interactions for maximizing cell degradation, representing the first cultured syntrophic Bacteroidota. We collectively revealed multiple symbiotic bacterial decomposition routes of dead prokaryotic cells, providing novel insight into the last step of the carbon cycle.
Volume 17(12)
Pages 2279-2289
Published 2023-12-1
DOI 10.1038/s41396-023-01538-2
PII 10.1038/s41396-023-01538-2
PMID 37872273
PMC PMC10689501
MeSH Anaerobiosis Archaea Bacteria* / genetics Bacteria* / metabolism Bacteria, Anaerobic* Environment
General Microbes JCM 15093 JCM 10133 JCM 15517 JCM 5805 JCM 1004 JCM 17603