論文 - 詳細
| RRC ID | 80508 |
|---|---|
| 著者 | Kammanee S, Muangham S, Butdee W, Klaysubun C, Igarashi Y, Duangmal K. |
| タイトル | Saccharopolyspora oryzae sp. nov., isolated from rhizosphere soil of the wild rice species Oryza rufipogon. |
| ジャーナル | J Antibiot (Tokyo) |
| Abstract |
A novel actinobacterium, designated as strain WRP15-2T, was isolated from rhizosphere soil of rice plant (Oryza rufipogon). The strain was Gram-stain-positive, aerobic, and non-motile. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain WRP15-2T fell into the genus Saccharopolyspora. The strain shared the highest 16S rRNA gene sequence similarity with the type strains Saccharopolyspora kobensis JCM 9109T (99.1%), Saccharopolyspora indica VRC122T (98.9%), and Saccharopolyspora antimicrobica DSM 45119T (98.7%). However, the digital DNA-DNA hybridization and average nucleotide identity values among these strains confirmed that the microorganism represented a novel member of the genus Saccharopolyspora. Chemotaxonomic data revealed that strain WRP15-2T possessed MK-9(H4) as the predominant menaquinone. It contained meso-diaminopimelic acid as the diagnostic diaminopimelic acid and arabinose, galactose, and ribose as predominant whole-cell sugars. The detected phospholipids were dominated by phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxy-phosphatidylmethylethanolamine, and phosphatidylcholine. The predominant cellular fatty acids were iso-C16:0, C16:0, and iso-C15:0. The G + C content of the genomic DNA was 69.5%. Based on these genotypic and phenotypic data, it is supported that strain WRP15-2T represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora oryzae sp. nov. is proposed. The type strain is WRP15-2T ( = TBRC 15728T = NBRC 115560T). |
| 巻・号 | 76(11) |
| ページ | 658-664 |
| 公開日 | 2023-11-1 |
| DOI | 10.1038/s41429-023-00647-6 |
| PII | 10.1038/s41429-023-00647-6 |
| PMID | 37596418 |
| MeSH | Bacterial Typing Techniques DNA, Bacterial / genetics Diaminopimelic Acid Fatty Acids Oryza* Phosphatidylethanolamines Phylogeny RNA, Ribosomal, 16S / genetics Rhizosphere Saccharopolyspora* / genetics Sequence Analysis, DNA Vitamin K 2 / analogs & derivatives |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 9109 JCM 16610 |