RRC ID 80561
Author Chen W, Zhang J-W, Qin B-X, Xie H-T, Zhang Z, Qiao X-Z, Li S-K, Asif M, Guo S, Cui L-X, Wang P-P, Dong L-H, Guo Q-G, Jiang W-J, Ma P, Xia Z-Y, Lu C-H, Zhang L-Q.
Title Quantitative detection of the Ralstonia solanacearum species complex in soil by qPCR combined with a recombinant internal control strain.
Journal Microbiol Spectr
Abstract DNA-based detection and quantification of soil-borne pathogens, such as the Ralstonia solanacearum species complex (RSSC), plays a vital role in risk assessment, but meanwhile, precise quantification is difficult due to the poor purity and yield of the soil DNA retrieved. The internal sample process control (ISPC) strain RsPC we developed solved this problem and significantly improved the accuracy of quantification of RSSC in different soils. ISPC-based quantitative PCR detection is a method especially suitable for the quantitative detection of microbes in complex matrices (such as soil and sludge) containing various PCR inhibitors and for those not easy to lyse (like Gram-positive bacteria, fungi, and thick-wall cells like resting spores). In addition, the use of ISPC strains removes additional workload on the preparation of high-quality template DNA and facilitates the development of high-throughput quantitative detection techniques for soil microbes.
Volume 11(6)
Pages e0021023
Published 2023-12-12
DOI 10.1128/spectrum.00210-23
PMID 37966217
PMC PMC10715031
MeSH DNA, Bacterial / analysis DNA, Bacterial / genetics Plant Diseases / microbiology Polymerase Chain Reaction / methods Ralstonia solanacearum* / genetics
General Microbes JCM 5969