| RRC ID |
80599
|
| 著者 |
Cho H, Ren Z, Divaris K, Roach J, Lin BM, Liu C, Azcarate-Peril MA, Simancas-Pallares MA, Shrestha P, Orlenko A, Ginnis J, North KE, Zandona AGF, Ribeiro AA, Wu D, Koo H.
|
| タイトル |
Selenomonas sputigena acts as a pathobiont mediating spatial structure and biofilm virulence in early childhood caries.
|
| ジャーナル |
Nat Commun
|
| Abstract |
Streptococcus mutans has been implicated as the primary pathogen in childhood caries (tooth decay). While the role of polymicrobial communities is appreciated, it remains unclear whether other microorganisms are active contributors or interact with pathogens. Here, we integrate multi-omics of supragingival biofilm (dental plaque) from 416 preschool-age children (208 males and 208 females) in a discovery-validation pipeline to identify disease-relevant inter-species interactions. Sixteen taxa associate with childhood caries in metagenomics-metatranscriptomics analyses. Using multiscale/computational imaging and virulence assays, we examine biofilm formation dynamics, spatial arrangement, and metabolic activity of Selenomonas sputigena, Prevotella salivae and Leptotrichia wadei, either individually or with S. mutans. We show that S. sputigena, a flagellated anaerobe with previously unknown role in supragingival biofilm, becomes trapped in streptococcal exoglucans, loses motility but actively proliferates to build a honeycomb-like multicellular-superstructure encapsulating S. mutans, enhancing acidogenesis. Rodent model experiments reveal an unrecognized ability of S. sputigena to colonize supragingival tooth surfaces. While incapable of causing caries on its own, when co-infected with S. mutans, S. sputigena causes extensive tooth enamel lesions and exacerbates disease severity in vivo. In summary, we discover a pathobiont cooperating with a known pathogen to build a unique spatial structure and heighten biofilm virulence in a prevalent human disease.
|
| 巻・号 |
14(1)
|
| ページ |
2919
|
| 公開日 |
2023-5-22
|
| DOI |
10.1038/s41467-023-38346-3
|
| PII |
10.1038/s41467-023-38346-3
|
| PMID |
37217495
|
| PMC |
PMC10202936
|
| MeSH |
Biofilms
Child
Child, Preschool
Dental Caries Susceptibility*
Female
Humans
Male
Selenomonas
Streptococcus mutans* / genetics
Virulence
|
| リソース情報 |
| 一般微生物 |
JCM 16777
JCM 12084 |
