| RRC ID |
80614
|
| Author |
Tochio T, Kawano K, Iyori K, Makida R, Kadota Y, Fujii T, Ishikawa H, Yasutake T, Watanabe A, Funasaka K, Hirooka Y, Nishifuji K.
|
| Title |
Topical erythritol combined with L-ascorbyl-2-phosphate inhibits staphylococcal growth and alleviates staphylococcal overgrowth in skin lesions of canine superficial pyoderma.
|
| Journal |
Pol J Vet Sci
|
| Abstract |
Erythritol (ERT) and L-ascorbyl-2-phosphate (APS) are bacteriostatic, but their effects on staphylococcal skin infections remain unknown. We aimed to determine whether ERT combined with APS inhibits the growth of staphylococci that are commonly isolated from pyoderma skin lesions in dogs. We investigated the individual and combined effects of ERT and APS on the growth of Staphylococcus pseudintermedius, S. schleiferi, and S. aureus using turbidity assays in vitro. Skin lesions from 10 dogs with superficial pyoderma were topically treated with 5% ERT and 0.1% APS for 28 days, and swabbed skin samples were then analyzed using 16S rRNA amplicon sequencing and quantitative real-time PCR (qPCR). Results showed that ERT inhibited S. pseudintermedius growth regardless of harboring the mecA gene, and APS increased the inhibitory effects of ERT against S. pseudintermedius, S. schleiferi, and S. aureus in vitro. Moreover, combined ERT and APS decreased the prevalence of staphylococci on canine skin lesions at the genus level. The combination slightly increased the α-diversity but did not affect the β-diversity of the microbiota. The qPCR results revealed that the combination significantly decreased S. pseudintermedius and S. schleiferi in skin lesions. Topical administration of EPS combined with APS can prevent staphylococcal colonization on the surface of mammalian skin. The results of this study may provide an alternative to systemic antibiotics for treating superficial pyoderma on mammalian skin surfaces.
|
| Volume |
26(4)
|
| Pages |
647-655
|
| Published |
2023-12-12
|
| DOI |
10.24425/pjvs.2023.148284
|
| PMID |
38088308
|
| MeSH |
Animals
Anti-Bacterial Agents / pharmacology
Anti-Bacterial Agents / therapeutic use
Dog Diseases* / drug therapy
Dogs
Mammals
Microbial Sensitivity Tests / veterinary
Pyoderma* / drug therapy
Pyoderma* / veterinary
RNA, Ribosomal, 16S
Staphylococcal Infections* / veterinary
Staphylococcal Skin Infections* / drug therapy
Staphylococcal Skin Infections* / veterinary
Staphylococcus aureus
|
| Resource |
| General Microbes |
JCM1751
JCM7470
JCM8703 |
