RRC ID 80625
Author Suzuki S, Yokota K, Igimi S, Kajikawa A.
Title Negative chemotaxis of Ligilactobacillus agilis BKN88 against gut-derived substances.
Journal Sci Rep
Abstract Ligilactobacillus agilis is a motile lactic acid bacterium found in the gastrointestinal tracts of animals. The findings of our previous study suggest that the motility of L. agilis BKN88 enables gut colonization in murine models. However, the chemotactic abilities of motile lactobacilli remain unknown. This study aimed to identify the gut-derived chemoeffectors and their corresponding chemoreceptors in L. agilis BKN88. Chemotaxis assays with chemotactic and non-chemotactic (ΔcheA) L. agilis strains revealed that low pH, organic acids, and bile salts served as repellents. L. agilis BKN88 was more sensitive to bile and acid than the gut-derived non-motile lactobacilli, implying that L. agilis might utilize motility and chemotaxis instead of exhibiting stress tolerance/resistance. L. agilis BKN88 contains five putative chemoreceptor genes (mcp1-mcp5). Chemotaxis assays using a series of chemoreceptor mutants revealed that each of the five chemoreceptors could sense multiple chemoeffectors and that these chemoreceptors were functionally redundant. Mcp2 and Mcp3 sensed all tested chemoeffectors. This study provides further insights into the interactions between chemoreceptors and ligands of motile lactobacilli and the unique ecological and evolutionary features of motile lactobacilli, which may be distinct from those of non-motile lactobacilli.
Volume 13(1)
Pages 15632
Published 2023-9-20
DOI 10.1038/s41598-023-42840-5
PII 10.1038/s41598-023-42840-5
PMID 37730901
PMC PMC10511705
MeSH Animals Bile Acids and Salts / pharmacology Biological Evolution Chemoreceptor Cells* Chemotaxis* Mice
General Microbes JCM 1048