| RRC ID |
80686
|
| Author |
Tada R, Higashidate T, Amano T, Ishikawa S, Yokoyama C, Kobari S, Nara S, Ishida K, Kawaguchi A, Ochi H, Ogino H, Yakushiji-Kaminatsui N, Sakamoto J, Kamei Y, Tamura K, Yokoyama H.
|
| Title |
The shh limb enhancer is activated in patterned limb regeneration but not in hypomorphic limb regeneration in Xenopus laevis.
|
| Journal |
Dev Biol
|
| Abstract |
Xenopus young tadpoles regenerate a limb with the anteroposterior (AP) pattern, but metamorphosed froglets regenerate a hypomorphic limb after amputation. The key gene for AP patterning, shh, is expressed in a regenerating limb of the tadpole but not in that of the froglet. Genomic DNA in the shh limb-specific enhancer, MFCS1 (ZRS), is hypermethylated in froglets but hypomethylated in tadpoles: shh expression may be controlled by epigenetic regulation of MFCS1. Is MFCS1 specifically activated for regenerating the AP-patterned limb? We generated transgenic Xenopus laevis lines that visualize the MFCS1 enhancer activity with a GFP reporter. The transgenic tadpoles showed GFP expression in hoxd13-and shh-expressing domains of developing and regenerating limbs, whereas the froglets showed no GFP expression in the regenerating limbs despite having hoxd13 expression. Genome sequence analysis and co-transfection assays using cultured cells revealed that Hoxd13 can activate Xenopus MFCS1. These results suggest that MFCS1 activation correlates with regeneration of AP-patterned limbs and that re-activation of epigenetically inactivated MFCS1 would be crucial to confer the ability to non-regenerative animals for regenerating a properly patterned limb.
|
| Volume |
500
|
| Pages |
22-30
|
| Published |
2023-8-1
|
| DOI |
10.1016/j.ydbio.2023.05.009
|
| PII |
S0012-1606(23)00093-3
|
| PMID |
37247832
|
| MeSH |
Animals
Animals, Genetically Modified
Epigenesis, Genetic*
Extremities* / physiology
Transcription Factors / genetics
Xenopus laevis / genetics
|
| IF |
2.896
|
| Resource |
| Clawed frogs / Newts |
Xenopus tropicalis strain Nigerian A (Xtr.NigerianAHuarc, RRID: HUARC_1001) |
