RRC ID 80714
Author Koyanagi T, Katayama T, Suzuki H, Kumagai H.
Title The LIV-I/LS system as a determinant of azaserine sensitivity of Escherichia coli K-12.
Journal FEMS Microbiol Lett
Abstract The growth of Escherichia coli is inhibited by an antibiotic compound, azaserine (O-diazoacetyl-L-serine). Previous studies revealed the biochemical properties of azaserine, which involves inhibition of various enzymatic reactions as well as introduction of DNA breakage. However, genetically, nothing has been elucidated except that all the azaserine-resistant strains isolated so far carry lesions in the aroP gene as a primary determinant. Here, we demonstrate that, in addition to AroP, the LIV-I/LS system, an ATP-binding cassette type transporter, is involved in azaserine sensitivity of E. coli, by genetic analysis and transport studies, in which Ki value for azaserine was determined to be approximately 10(-3) M.
Volume 237(1)
Pages 73-7
Published 2004-8-1
DOI 10.1016/j.femsle.2004.06.017
PII S0378109704004367
PMID 15268940
MeSH Amino Acid Transport Systems / genetics* Amino Acid Transport Systems / metabolism* Anti-Bacterial Agents / metabolism Anti-Bacterial Agents / pharmacology Azaserine / metabolism* Azaserine / pharmacology* Escherichia coli / drug effects* Escherichia coli / growth & development Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Gene Deletion Genes, Bacterial Genetic Complementation Test Leucine / metabolism Membrane Transport Proteins / genetics Membrane Transport Proteins / metabolism Microbial Sensitivity Tests Phenylalanine / metabolism
IF 1.987
Resource
Prokaryotes E. coli ME9987 ME9988 ME9989