RRC ID 80716
Author Kurihara S, Oda S, Kumagai H, Suzuki H.
Title Gamma-glutamyl-gamma-aminobutyrate hydrolase in the putrescine utilization pathway of Escherichia coli K-12.
Journal FEMS Microbiol Lett
Abstract gamma-Glutamyl-gamma-aminobutyrate hydrolase (PuuD) was purified and the properties of the enzyme were characterized. The active center of PuuD was identified as Cys-114 by site-directed mutagenesis. The expression of PuuD was induced by putrescine and O2 (substrates of the Puu pathway), while the addition of succinate or NH4Cl (products of the Puu pathway) to the medium reduced the expression of PuuD. The findings that the puuD-deficient strain accumulated gamma-glutamyl-gamma-aminobutyrate (gamma-Glu-GABA) and could not grow on putrescine as a sole nitrogen source indicate that PuuD is physiologically important as a gamma-Glu-GABA hydrolase.
Volume 256(2)
Pages 318-23
Published 2006-3-1
DOI 10.1111/j.1574-6968.2006.00137.x
PII FML137
PMID 16499623
MeSH Aldehyde Oxidoreductases / genetics Aldehyde Oxidoreductases / isolation & purification* Aldehyde Oxidoreductases / metabolism Ammonium Chloride / pharmacology Binding Sites / genetics Dipeptides / metabolism Escherichia coli K12 / enzymology* Escherichia coli K12 / genetics Escherichia coli K12 / growth & development Escherichia coli K12 / metabolism Gene Expression Regulation, Bacterial Mutagenesis, Site-Directed Nitrogen / metabolism Oxygen / pharmacology Putrescine / metabolism* Putrescine / pharmacology Succinic Acid / pharmacology
IF 1.987
Resource
Prokaryotes E. coli ME10006