RRC ID 80722
Author Tawara H, Tsunematsu T, Kitajima S, Nagao R, Matsuzawa S, Otsuka K, Ushio A, Ishimaru N.
Title The noncanonical function of borealin, a component of chromosome passenger complex, promotes glycolysis via stabilization of survivin in squamous cell carcinoma cells.
Journal Biochem Biophys Res Commun
Abstract The chromosome passenger complex (CPC) is a kinase complex formed by Aurora B, borealin, survivin and inner centromere protein (INCENP). The CPC is active during mitosis and contributes to proper chromosome segregation via the phosphorylation of various substrates. Overexpression of each CPC component has been reported in most cancers. However, its significance remains unclear, as only survivin is known to confer chemoresistance. This study showed that the overexpression of borealin, a CPC component, stabilized survivin protein depending on its interaction with survivin. Unexpectedly, the accumulation of survivin by borealin overexpression did not affect the well-characterized functions of survivin, such as chemoresistance and cell proliferation. Interestingly, the overexpression of borealin promoted lactate production but not the overexpression of the deletion mutant that lacks the ability to bind to survivin. Consistent with these findings, the expression levels of glycolysis-related genes were enhanced in borealin-overexpressing cancer cells. Meanwhile, the overexpression of survivin alone did not promote lactate production. Overall, the accumulation of the borealin-survivin complex promoted glycolysis in squamous cell carcinoma cells. This mechanism may contribute to cancer progression via excessive lactate production.
Volume 706
Pages 149741
Published 2024-4-30
DOI 10.1016/j.bbrc.2024.149741
PII S0006-291X(24)00277-8
PMID 38471204
MeSH Aurora Kinase B / genetics Aurora Kinase B / metabolism Carcinoma, Squamous Cell* / genetics Cell Cycle Proteins / metabolism Centromere* / metabolism Chromosomal Proteins, Non-Histone / metabolism Humans Lactates Mitosis Phosphorylation Survivin / genetics Survivin / metabolism
Resource
DNA material CSII-CMV-MCS-IRES2-Bsd (RDB04385) pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394)