論文 - 詳細
| RRC ID | 80734 |
|---|---|
| 著者 | Liu C, Rex R, Lung Z, Wang JS, Wu F, Kim HJ, Zhang L, Sohn LL, Dernburg AF. |
| タイトル | A cooperative network at the nuclear envelope counteracts LINC-mediated forces during oogenesis in C. elegans. |
| ジャーナル | Sci Adv |
| Abstract |
Oogenesis involves transduction of mechanical forces from the cytoskeleton to the nuclear envelope (NE). In Caenorhabditis elegans, oocyte nuclei lacking the single lamin protein LMN-1 are vulnerable to collapse under forces mediated through LINC (linker of nucleoskeleton and cytoskeleton) complexes. Here, we use cytological analysis and in vivo imaging to investigate the balance of forces that drive this collapse and protect oocyte nuclei. We also use a mechano-node-pore sensing device to directly measure the effect of genetic mutations on oocyte nuclear stiffness. We find that nuclear collapse is not a consequence of apoptosis. It is promoted by dynein, which induces polarization of a LINC complex composed of Sad1 and UNC-84 homology 1 (SUN-1) and ZYGote defective 12 (ZYG-12). Lamins contribute to oocyte nuclear stiffness and cooperate with other inner nuclear membrane proteins to distribute LINC complexes and protect nuclei from collapse. We speculate that a similar network may protect oocyte integrity during extended oocyte arrest in mammals. |
| 巻・号 | 9(28) |
| ページ | eabn5709 |
| 公開日 | 2023-7-14 |
| DOI | 10.1126/sciadv.abn5709 |
| PMID | 37436986 |
| PMC | PMC10337908 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins* / genetics Cell Nucleus Laminin Mammals Nuclear Envelope* Oocytes Oogenesis / genetics |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 28 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1651 |