RRC ID 80760
Author Higurashi S, Tsukada S, Aleogho BM, Park JH, Al-Hebri Y, Tanaka M, Nakano S, Mori I, Noma K.
Title Bacterial diet affects the age-dependent decline of associative learning in Caenorhabditis elegans.
Journal Elife
Abstract The causality and mechanism of dietary effects on brain aging are still unclear due to the long time scales of aging. The nematode Caenorhabditis elegans has contributed to aging research because of its short lifespan and easy genetic manipulation. When fed the standard laboratory diet, Escherichia coli, C. elegans experiences an age-dependent decline in temperature-food associative learning, called thermotaxis. To address if diet affects this decline, we screened 35 lactic acid bacteria as alternative diet and found that animals maintained high thermotaxis ability when fed a clade of Lactobacilli enriched with heterofermentative bacteria. Among them, Lactobacillus reuteri maintained the thermotaxis of aged animals without affecting their lifespan and motility. The effect of Lb. reuteri depends on the DAF-16 transcription factor functioning in neurons. Furthermore, RNA sequencing analysis revealed that differentially expressed genes between aged animals fed different bacteria were enriched with DAF-16 targets. Our results demonstrate that diet can impact brain aging in a daf-16-dependent manner without changing the lifespan.
Volume 12
Published 2023-5-30
DOI 10.7554/eLife.81418
PII 81418
PMID 37252859
PMC PMC10229132
MeSH Aging / physiology Animals Caenorhabditis elegans* / physiology Caenorhabditis elegans Proteins* / genetics Diet Escherichia coli / physiology Forkhead Transcription Factors / genetics Longevity / physiology
C.elegans tm4529