論文 - 詳細
| RRC ID | 80764 |
|---|---|
| 著者 | Jafari G, Khan LA, Zhang H, Membreno E, Yan S, Dempsey G, Gobel V. |
| タイトル | Branched-chain actin dynamics polarizes vesicle trajectories and partitions apicobasal epithelial membrane domains. |
| ジャーナル | Sci Adv |
| Abstract |
In prevailing epithelial polarity models, membrane- and junction-based polarity cues such as the partitioning-defective PARs specify the positions of apicobasal membrane domains. Recent findings indicate, however, that intracellular vesicular trafficking can determine the position of the apical domain, upstream of membrane-based polarity cues. These findings raise the question of how vesicular trafficking becomes polarized independent of apicobasal target membrane domains. Here, we show that the apical directionality of vesicle trajectories depends on actin dynamics during de novo polarized membrane biogenesis in the C. elegans intestine. We find that actin, powered by branched-chain actin modulators, determines the polarized distribution of apical membrane components, PARs, and itself. Using photomodulation, we demonstrate that F-actin travels through the cytoplasm and along the cortex toward the future apical domain. Our findings support an alternative polarity model where actin-directed trafficking asymmetrically inserts the nascent apical domain into the growing epithelial membrane to partition apicobasal membrane domains. |
| 巻・号 | 9(26) |
| ページ | eade4022 |
| 公開日 | 2023-6-28 |
| DOI | 10.1126/sciadv.ade4022 |
| PMID | 37379384 |
| PMC | PMC10306301 |
| MeSH | Actins* Animals Caenorhabditis elegans Caenorhabditis elegans Proteins* / genetics Cell Membrane Intestines |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.2 |
| リソース情報 | |
| 線虫 | tm6888 tm6924 |